This study was performed to explore the effect and significance of gastric mucosal histology and ITF mRNA in stress-related rats with lectroacupuncture (EA) on the stomach channel and gallbladder channel. Forty rats were randomly divided into 4 groups: blank control group, model control group, Stomach Meridian of Foot-Yangming(SMFY) group, Gallbladder Meridian of Foot-Shaoyang (GMFY) group. After some procedures, all rats (except normal group) were made model by WRS on day 7. Then the gastric mucosa tissure of each rat was taken off after assessment of gastric mucosal lesion index(GUI), and half of above tissue in each group of was detected the expression of ITF mRNA by Reverse-transcriptase- polymerase chain reaction(RT-PCR) method, meanwhile the gastric mucosal ulcer index（UI） and the histological change and degree of injury of gastric mucosa were observed grossly under light microscope or magnifier. As a result, the UI value in SMFY/ GMFY group decreased obvious or extremly obvious（P﹤ 0.05 or 0.01）; EA had a tendency of improving: The level of ITF mRNA expression of SMFY group was strongly higher than that of model group(P﹤0.01), while that of GMFY group was high than model group but no statistic difference(P﹥0.05); Mucosal hyperaemia and edema but little hemorrhage and gastric ulcer could be observed in group with EA under light microscope, and that of SMFY group was more significant than GMFY. The difference effect of gastric mucosal protection by EA on stomach channel and gallbladder channel, was related to the expression variance of intestinal trefoil factor.

Objective：To study the effect of medicinal herb-cake-separated moxibustion on serum lipoprotein levels in hyperlipemia rabbits. Methods: A total of 55 New-Zealand rabbits were randomly divided into control group (n=13), model group (n=14) and medicinal herb-cake-separated moxibustion (indirect moxibustion) group (n=14). Hyperlipemia model was established by feeding the animals with specialized forage (15% vitellus powder, 5% cholesterol and common forage )for 6 weeks. Moxibustion was applied to “Juque”(CV14), “Tianshu”(ST40), etc, 4 moxa-cones for every acupoint, once daily and continuously for 40 days. Serum triglyceride (TG) and total cholesterol (TCH) contents were assayed with colorimentric method. Results:Compared with control group, TCh and TG levels of model group increased significantly (p<0.01). TCh and TG contents of direct moxibustion and indirect moxibustion groups were significantly lower than those of model group (p<0.01).Comparison between two moxibustion groups showed that serum TCh level of indirect moxibustion group was strikingly lower than that of direct moxibustion group (p<0.01). It indicated that both direct and indirect moxibustion could effectively lower hyperlipemia and the therapeutic effect of indirect moxibustion was significantly superior to that of direct moxibustion in lowering serum TCh level. Conclusion: Both direct and indirect moxibustion canregulate lipid metabolism and the therapeutic effect of medicinal herb-cake-separated moxibustion is superior to that of direction moxibustion in hyperlipemia rabbits.

目的：观察电针胃经（穴）对应激性胃溃疡大鼠胃黏膜细胞蛋白激酶C活性的影响。方法：实验于2005-07/12在湖南中医药大学针灸基础实验室完成。选择健康SD大鼠60只，随机数字表法分为5组，胃溃血清组（12只）、胃经血清组（12只，取穴；四白、梁门、足三里）和胆经血清组（12只，取穴：阳白、日月、阳陵泉）、胃经血清+PD153035组（12只）和胆经血清+PD153035组（12只）。采用水浸束缚法制作胃溃疡动物模型。电针刺激用G6805型电针仪，疏密波，电针频率疏波4Hz，密波20Hz，强度以肌肉或针柄微颤为度，电针时间30min。利用链酶蛋白酶消化法分离胃黏膜细胞，分别用表皮生长因子受体抑制剂PD153035和血清孵育胃黏膜细胞，应用同位素掺人法检测蛋白激酶C活性。结果：纳入动物60只，均进入结果分析。溃疡血清大鼠胃黏膜细蛋白激酶C有少量表达[（29.16±30.94）nkat/g]；胃经血清组和胆经血清组大鼠胃黏膜细胞蛋白激酶C呈现较强上增性表达，其中胃经血清组大鼠上增性表达特别明显，两组相比差异有显著性意义[分别为（114.92±65.61），（36.47±27.54）nkat/g，P<0.01]；胃经血清+PD153035组和胆经血清+PD153035组大鼠胃黏膜细胞蛋白激酶C可见微弱表达[分别为（17.39±23.39），（23.44±14.42）nkat/g]，胃经血清组胃经血清+PD153035组比较差异有显著性意义（p<0.01）。结论：针刺能刺激大鼠胃黏膜细胞信号转导通路中蛋白激酶C的活化，并且存在经脉-脏腑的特异性联系。

expression in gastric mucosal cells. Such tendency existed in the model group (0.7104 ± 0.0457) but with no signifi cant difference (P = 0.495 > 0.05) and in model serum group (0.8516 ± 0.0409) with an extremely obvious difference (P = 0.001 < 0.01). Furthermore, the EGFR gene expression in stomach serum group was significantly higher than that in gallbladder serum group (P = 0.00<0.01). CONCLUSION: The present study shows that serum derived from rats treated with electroacupuncture atstomach meridian acupoints can distinctly increase the EGFR gene expression of gastric mucosal cells. Therefore, there is certain meridian specificity in the serum, which could provide a proof for the TCM theory “particular relation between meridian and internal organ”.

目的：观察针刺胃经穴后的血清对胃溃疡大鼠胃黏膜细胞磷脂酶Cγ-1（PLCγ-1）活性的影响。方法：大鼠60只随机分为溃疡血清组、胃经血清组、胆经血清组、胃经血清+PD153035组和胆经血清+PD153035组，采用水浸束缚法制作胃溃疡模型，利用链霉蛋白酶消化法分离胃黏膜细胞，分别用表皮生长因子受体（EGFR）抑制剂PD153035和血清孵育胃黏膜细胞，应用酶联免疫吸附（ELISA）法检测PLCγ-1活性.结果：胃经血清组（13.0±7.5 nkat/g）大鼠胃黏细胞PLCγ-1活性与溃疡血清组（6.5±0.3nkat/g）比较有显著性差异（p=0.01）；胃经血清组大鼠胃粘膜细胞PLCγ-1活性与胆经血清组（7.2±1.8nkat/g）比较有显著性差异（p=0.02）；胃经血清组大鼠胃黏膜细胞PLCγ-1活性与胃经血清+PD153035组（7.4±2.7nkat/g）比较有显著性差异（p=0.02）。结论：针刺胃经穴后的血清能刺激胃黏膜细胞中PLCγ-1的活化，并且存在经脉-脏腑的特异性联系。