The present study reported the ionic liquid (IL) used as running electrolyte in capillary zone electrophoresis (CZE) with β-cyclodextrin (β-CD) as modifier for the separation of anthraquinones extract of Chinese herb Paedicalyx attopevensis Pierre ex Pitard. The optimum running electrolyte was 60mM 1-butyl-3-methylimizolium tetrafluoroborate (1B-3MI-TFB) solution with 4.0mM β-CD. The pH was 10.00 and the applied voltage was 20 kV with detection at 254nm. The present method was compared with others and the effect of Joule heating was discussed as well. More significantly, this method is the development of the ionic liquids application to the capillary electrophoresis.

A high-performance liquid chromatographic (HPLC) method is described for the simultaneous determination of honokiol and magnolol in rat plasma. The plasma was deproteinized with acetonitrile which contained an internal standard (diphenyl) and was separated from the aqueous layer by adding sodium chloride. Honokiol and magnolol are extracted into the acetonitrile layer with high yield, and determined by reversed-phase HPLC and ultraviolet detection. The limits of quantitation for honokiol and magnolol were 13 and 25 ng ml 1 in plasma, respectively, and recovery of both analytes was greater than 93%. The assay was linear from 20 to 200ngml. for honokiol and from 40 to 400ngml-1 for magnolol. Variation over the range of the standard curve was less than 15%. The method was used to determine the concentration-time profiles of honokiol and magnolol in the plasma following rectal administration of Houpo extract at a dose of 245 mg kg-1, equivalent to 13.5, 24.4mg kg-1 of honokiol and magnolol, respectively.

This is the first report on the determination of proteins with 3-hydroxy-4-(2-hydroxy-4-sulfo-1-naphthalenyl)azo (Cal-Red) by Rayleigh light scattering (RLS). At pH 4.07, the weak RLS of Cal-Red can be enhanced greatly by the addition of proteins. On this basis, the reaction of Cal-Red and proteins was studied. A new quantitative determination method for proteins has been developed. This method is very sensitive (0:45-36:9lgml 1 for bovine serum albumen (BSA)), rapid (<2 min), simple (one step), and tolerant of most interfering substances. The maximum binding number of Cal-Red to BSA was 143 and the binding constant was 4.1

A new method is proposed for the spectrophotometric determination of Pd(Ⅱ), based on the reaction of Pd(Ⅱ) with 2-(4-chloro-2-phosphonophenylazo)-7-(3-carboxyphenylazo)-1,8-dihydroxynaphthalene-3,6-disulfonic acid(CPA-mK) in sulfuric acid without heating. Beer's law is obeyed for 1.0-4.0 mg of Pd (Ⅱ) in 10 mL of solution. The calibration curve from 1.0 to 42.0mg in 10mL of solution is modeled successfully by artificial neural networks (ANNs). The maximum relative error between experimental values and the values predicted by ANNs is 1.5%. In comparison with some mathematical functions, ANNs show better ability for curve fitting, thus greatly extending the applicable range of the calibration curve of this new system. The method has been applied to determine Pd (Ⅱ) in ore and catalyst samples with a relative error of less than 4% and with a recovery range between 94% and 103%.

A new rapid, selective and sensitive on-line microwave flow injection-kinetic method was developed for spectrophotometric determination of micro amounts of Ir(Ⅲ), based on its catalytic effect on the m-acetylchlorophosphonazo (CPA-mA) and KIO4 reaction in NaOH media. An on-line microwave oven was employed to accelerate the reaction. The reaction was followed spectrophotometrically by measuring the decrease of the absorbance of CPA-mA at 580 nm. The effect of five variables for the determination of Ir(Ⅲ) was optimized by means of a multilayer artificial neural network using extended deltabar-delta (EDBD) algorithms. Under the optimum experimental conditions, Ir(Ⅲ) could be determined in the range 0.060-0.60 μgZZZ; mL-1 with detection limit of 0.02 μgZZZ;mL-1 and the sampling frequency of 34 h-1. The proposed method was applied to the determination of micro amounts of Ir(Ⅲ) in refined ore and secondary alloy with the recoveries from 91.4% to 109%.