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【期刊论文】XANTttAN GUM PRODUCTION WITH PUMPING STATIC-MIXING LOOP FERMENTOR (PS-LOOP FERMENTOR)
许平, Xu Ping, Lin Jianqiang, Lin Jianqun and Jiang Boying
Vohune 16 No.5 (May 1994) pp. 523~526,-0001,():
-1年11月30日
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许平, P. XU*, , A. THOMAS, and C. D. GILSON
Volume 18 No.12 (December 1996) pp. 1439~1440,-0001,():
-1年11月30日
Ethanol concentration and fermentation productivity using Saccharomyces cerevisiae were substantially increased in shake flask cultures with a normal inoculum by combining 3 methods: (a) by making nutrient additions to the standard medium for ethanol production, (b) by immobilizing the cells in alginate beads and (c) by using a glucose step-feeding batch process. Ethanol concentration by free yeast was improved from 5.9% (w/w) to 9.6% (w/w) when a further 0.8% yeast extract and 1% animal peptone were added to the standard 30% (w/v) glucose nutrient medium. This was further increased to 12.8% (w/w) by using alginate immobilized yeast. The ethanol concentration was increased again, to 15.0% (w/w) by using the glucose step-feeding batch process.
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【期刊论文】Characterization of a Lactate Oxidase from a Strain of Gram Negative Bacterium from Soil
许平, PING XU, , TOSHIHIRO YANO, KENJI YAMAMOTO, HIDEYUKI SUZUKI, AND HIDEHIKO KUMAGAI*
,-0001,():
-1年11月30日
A lactate oxidase was purified about 36-fold from a newly screened strain KY6 of gram negative bacterium from soil to yield a homogeneous protein. The native enzyme had a molecular mass of 204 kDa easured by Sephadex G-200 and that of subunit on the SDS-PAGE was found to be 45 kDa. The enzyme was optimally active at pH 7.7 and showed stability at pH range of 5.7 to 9.5 for 24 h at 4℃. The optimum temperature was 70℃ and the enzyme activity was stable for 10min up to 45℃. The half-life of the enzyme activity was about 10 min at 55℃. The best substrate of the enzyme was D-lactate and Km value for D-lactate was 0.14raM. The Km value for DL-lactate was 0.20mM. Substrate inhibition of the enzyme was observed at higher concentrations than 20mM of DL-lactate and 10mM of D-lactate.
Lactate oxidase, characterization, bacterium, pyruvate preparation.,
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