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郑筱祥, XIAOCHUN CHEN, CHAO SHENG and XIAOXIANG ZHENG*
Cell Biology International 2001, Vol. 25, No.7, 593-598,-0001,():
-1年11月30日
A method for the direct measurement of intracellular nitric oxide (NO) production stimulated by penicillin G (PG) in cultured hippocampal neurons with diaminoanthraquinone (DAA) using laser scanning confocal microscopy (LSCM) was developed. Intracellular DAA fluorescence could specifically represent NO production based on two facts: (1) 3-morpholinosydnonimine, a NO donor, could dose-dependently increase DAA fluorescence; and (2) haemoglobin, a NO scavenger, could inhibit the increase of DAA fluorescence. The PG dose-dependently increasd the intercellular level of glutamate (Glu, 5 min after stimulation) and the intracellular NO production (30 min throughout stimulation). The increase of NO production could be reversed by Nw-nitro-L-arginine (a NO synthase inhibitor), and also by D(-)2-amino-5-phosphonovaleric acid, a subtype of Glu receptor antagonist. These results revealed that DAA could be used to indicate real-time and kinetic intracellular NO production of hippocampal neurons with higher sensitivity, specificity and accuracy.
nitric oxide, confocal microscopy, diaminoanthraquinone, epilepsy, hippocampal neurons, penicillin G, glutamate.,
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郑筱祥, Mu Zhang, Gangmin Ning, Caihua Shou, Yinjun Lu, Xiaoxiang Zheng
Zhang M et al. Inhibitory Effect of. Planta Med 2003: 69: 692-695,-0001,():
-1年11月30日
Jujuhoside A (JUA) is a main component of jujubogenia exrracted from the seed of Ziziphus jujuba Mill var spinosa (Bunge) Hu ex H F Chou (Ziziphus), which is widely used in Chinese traitional metiicine for the treatment of insomnia and anxiety. Previously, we reported the inhibitinry effects of JuA on hippocampal forma-tion in vivo and in vitro, the present study was carried out to ex amine the effects of JuA on glutamate (Glu)-mediated excitatory signal pathway in hippocamput. Microdialysis coupled with high-performance Liquid chromatography (HPLC) was used to monitor the changes of Glu Levels in the hippocampus induced by penicillin sodium, or a mixture of penicillin sodium and JuA. The results showed that penicillin increased the hippocampal GlU concentration (p<0.Ol) and a high dose of JuA (0.1g/L) sig-mificantly blocked penicillin-indued Glu release (P<0.05). Moreover, the effect of JuA on intracellular Ca2+ changes after the stimulation by Glu was studied in cultured hippocampal neurons with can focal laser scanning microscope (CLSM). It was found that Clu (O.5 mM) reduced an intracellular [Ca2+], increase (p<0.01), and JuA significantly inhibited the Glu-induced Ca2+. increase. The calmodulin (CAM) antatgonist trifluoperazine (TFP) showed a similar inhibitory effect as JuA. These observations suggested tbat JuA has inhibitory effects on Glu-mediated excita-tory signal pathway in hippocampus and probably acts through its anti-calmodulin action
Jujuboside A,, glutamate-calcium,, inhibitory effect,, hippocam-pus,, Ziziphus jujuba,, Rhammaceae
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郑筱祥
,-0001,():
-1年11月30日
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郑筱祥, Xiao-Long Li, Wei-Min Yah, Hai-Yam Li, Hao Xu, Xiao-Xiang Zheng, Dian-Wu Guo, Li-Ke Tanh
Li X-L et al. The Study of. Planta Med 2004: 720: 1039-1044,-0001,():
-1年11月30日
Brevlseapine is a flavonoid extracted from Engeron breviscapus. Hand. Mazz, and it has been reported that breviscapine can actirate K+ channels and block Ca2+ channels, In this paper, we stud-led the cardloprotective effects of breviscapine on elect rocardio gram (ECG) changes (ST segment elevation), infarction size in dog heart subjected to myocardial infarction caused by left co-onacy artery ligation and lactate dehydrngenase (LDH) leakage. changes of [ntracellular free Ca2+ levels, apoptosis and necrosis in cultured neonatal rat cardiomyocytes subjected to hypoxia. Adidonally, the effect of breviscapine on myocardial oxygen consumption was detected in dog myocardium in vitro. The re suits showed that breviscapine treatment (1mg/kg, 2 mg/kg and 4mg/kg) significantly reduced ST-segment elevation and in-farction size in hearts subjected to myocardial infarction, that breviscapine treatment (1429ug/mL, 28.57ug/m L and 57.14ug/mL) significantly decreased oxygen consumption in myoear-dium, and that breviscapine treatment (5 Ug/mL. 10Ug/mL and 20Ug/mL) significantly reduced LDH leakage, intracellular free Ca2+levels, apoptosis and necrosis in cardiomyocytes subjected to hypoxia. In conclusion, the present study indicates that blevis-capine is in favor of myocardial protection.
Breviseapine flavone,, myocardial infarction
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郑筱祥
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-1年11月30日
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