A novel method based on flow cytometry (FCM), which can count the number of detected cells, has been developed for the evaluation of cellular proliferation and cytotoxicity in vitro. It provides a tool that directly counts cell number without being influenced by the metabolic state of the cells, discriminates target cells from effector cells in cell-mediated cytotoxicity assay, and with less treatment step and free radioactivity. In this paper, we have prepared the PG cells (a highly metastatic human lung cancer cell line) and peripheral blood lymphocytes (PBL) with various concentrations and ratios of concentration to validate the method. The results were compared with MTT (3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide) assay and the regression analysis results showed that this method worked very well. We have also used this method to evaluate mitogeninduced proliferation and cytotoxicity. The results indicated that this method might yield high sensitivity and reliability.

目的:观察中药酸枣仁皂俄A对青霖素钠诱导产生的大鼠海马脑片CAI区兴奋性放电的抑制作用。方法：细胞外记录离体大鼠海马脑片CAI区锥体细胞层群体峰电位.结果:青霉素钠500，100和200Ku/L可剂量依赖地诱导海马脑片上CAI区神经元的兴奋苯巴比妥钠0.02-0.05g/L和酸枣仁皂成A0.05-0.10g/L都可以剂量依赖性地抑制这种青霉素钠诱发的兴奋反应.结论:高剂量的酸枣仁皂试A能够抑制青霉素钠诱导的海马CAI区兴奋性电位.群峰电位（PS）的个数和第一个峰电位的幅度受到的抑制较明显，而兴奋性突触后场电位的变化不大。

Confocal laser scanning microscopy (CLSM) is one of the common equipments being used to observe the intracellular substances like nitric oxide (NO) and calcium ions (Ca2). The intracellular substances detection relies on the proper analysis of the fluorescence images obtained by CLSM. At present, the outlines of the cells in CLSM images from fluorescence are manually drawn by judging the contrast between the object and the background. This method is subjective and may have the possibility of inaccuracy. In this paper we have designed a technique based on digital imaging processing to automatically detect the outlines or contours of the cells and assess the production of NO labeled by fluorescence probes. The developed technique was tested on a series of images obtained from CLSM and the spread ofNO during the time course was satisfactorily estimated.

建立一个用于体外研究特定流动剪切力作用下白细胞和内皮细胞动态相互作用的方法。利用建立的平板流动小室系统可在体外产生特定的流动剪切力。将培养的人脐静脉内皮细胞装入平板流动小室后，以0.71dynes/cm2的流动剪切力把含有吖啶橙染色的白细胞的灌流液导入流动小室，由此产生了白细胞和内皮细胞的动态粘附过程。整个粘附过程通过OlympusIX70倒置荧光显微系统观察，同时通过CCD摄象头录像。然后用图象采集卡将录像采集为数字图象并保存。利用针对实验设计的图象处理和分析方法，对采集的数字图象进行处理和测量，可以得到粘附白细胞的个数和滚动白细胞的速度。通过研究内毒素脂多糖（LPS）对内皮细胞粘附功能的促进及地塞米松（DXM）对该刺激的抑制作用来验证。对于用内毒素脂多糖（LPS）处理的内皮细胞，固定粘附和慢速滚动的白细胞的个数比对照组分别显著增加了23.7倍和4.1倍，同时白细胞在粘附作用过程中慢速滚动和快速滚动的速度比对照组明显降低了25.6%和26。1%而对于脂多糖和地塞米松（DXM）处理过的内皮细胞，上述内毒素引起的影响被显著抑制了。该方法可以用于研究不同的化学和物理刺激对内皮细胞功能的影响机制，及用来评价各类抑制内皮细胞粘附功能的药物。