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【期刊论文】a-Lactalbumin Mutant Acting as Lysozyme
朱德煦, Yuming Xue, Jian-Ning Liu, , Ziyong Sun, Zhong Ma, Chunlei Wu, and Dexu Zhu* State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, Nanjing University, Nanjing, People's Republic of China; Institute of Molecular Medicine, People's Republic of China
PROTEINS: Structure, Function, and Genetics 42: 17-22 (2001),-0001,():
-1年11月30日
A mutant of a-lactalbumin was expressed and purified, in which His32, Thr33, Glu49, Ile59, Val99, and Tyr103 were substituted by Leu32, Glu33, Asp49, Trp59, Asn99, and Ala103, respectively, to create a catalytic site of lysozyme in a-lactalbumin. The mutant catalyzed hydrolysis of the synthetic substrate, pNP-(NAcGlc)3, with a KM and kcat of 0.160
a-lactalbumin, chicken lysozyme, chitopentaose, glycosidic bond, isothermal titration calorimeter, pNP-(, NAcGlc), 3
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朱德煦, Ni Cheng, a Jian-Shu Xie, b Min-Yue Zhang, a Chang Shua and De-Xu Zhua, *
Bioorganic & Medicinal Chemistry Letters 13(2003)2703-2707,-0001,():
-1年11月30日
The synthesis and anti-Helicobacter pylori activity of a novel agent NE2001, 4-(4-methylbenzyl)-40-[guanidinomethylbenzoyloxy] biphenyl-4-carboxylate hydrochloride, are described. NE2001 had a specific inhibitory effect on the growth of H. pylori preceded by the suppression DNA synthesis in the cell. The effects of NE2001 on RNA and protein syntheses in H. pylori were also examined.
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【期刊论文】Renaturation of Recombinant Human Pro-Urokinase Expressed in Escherichia coli
朱德煦
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-1年11月30日
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