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【期刊论文】Seasonal changes in phospholipids ofmussel (Mytilus edulisLinne)
林洪, Hong Lin, Jie Jiang, * Chang-hu Xue, Bin Zhang and Jia-Chao Xu
Feuenal of the Science of Food and Agrienlture 83: 133-135(online: 2003),-0001,():
-1年11月30日
The phospholipids of mussels (Mytilus edulisl Linne) from the coast of Qingdao were extracted, fractionated and analysed over a 12 month period. Thecontents of total lipids, neutral lipids, polar lipids and phospholipids were meausred. The composition of phosholipids was determined by high-performance liquid chromatiorgraphy, and their fatty acid composition was analysed by gas chromatorgraphy. Thephospholipide contert ranged from 3.6 to 6.4 g kg-1 soft tissue. Pe (phosphatidyl ethanlaminc) and PC (phosphatidyl chline) were the major constituents. C16:0, C20:5 and C22:6 wree the major fatty acids. C20:5 (5.25-23.10%) and C22:6 (6.05-20.42%) varied regularyly with the seasonal factors, Their total amounts were high from January to Juhne, which wouldbe an optimal time for the utilisation of trhe phospholipids of mussels.
phospholipid, mussel, seasonal change, eicosapentaenoic acid (, EPA), , docosahexaenoicacid (, DHA),
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林洪, Wang Weifena, *, Lin Honga, Xue Changhua, Khalid Jamilb
Environment International 30(2004)367-373,-0001,():
-1年11月30日
Muscle residue depletion of chloramphenicol (CAP), sulphamethoxazole (SMZ) and oxytetracycline (OTC) following oral administration was evaluated in shrimp, Penaeus chinensis under field conditions. Three groups of shrimps were cultured in tanks filled with seawater and fed a commercial medicated diet containing 2000 mg kg 1 CAP, SMZ and OTC, respectively, twice daily for 3 days. Sampling was conducted at different intervals (0, 0.5, 1, 1.5, 2, 3, 4, 8, 10, 12, 16, 24, 36, 48, 72, 96, 120, 144, 168 h) after the cessation of medication. Drug analysis was carried out by HPLC. The elimination half-life (t1/2) of CAP was 10.04 h, the elimination half-life (t1/2) of SMZ was 5.68 h and that of OTC was 16.12 h. If the EU MRPL value of 0.3 ng/g for CAP and MRL value of 0.1 Ag/g for SMZ and OTC quoted for muscle from finfish is extended to shrimp muscle, extrapolation of the data indicates that it would be passed after a 139.7 h (95% CI=132.0-144.4 h), 30.6 h (95% CI=27.2-33.1h), 90.3 h (95% CI=87.9-92.5h) withdrawal period for CAP, SMZ and OTC in shrimp muscle, respectively.
Elimination, Chloramphenicol, Sulphamethoxazole, Oxytetracycline, Shrimp Penaeus chinensis
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【期刊论文】Preparation of chitosan oligomers by immobilized papain
林洪, Hong Lin* Haiying Wang, Changhu Xue, Mei Ye
Enzyme and Microbial Technology 31(2002)588-592,-0001,():
-1年11月30日
Immobilized papain was used to prepare chitosan oligomers and the optimum conditions were as follows: NaAc-HAc buffer system, pH 4.0, temperature 45℃. After 24 h reaction with stirring, nanofiltration and ultrafiltration were used to desalt and isolate chitosan oligomers into different molecular weight fractions respectively. The yield of chitosan oligomers with molecular weight under 10,000 was 49.55% to total substrate, while that of between 600 and 2000 was 11.07%. The fractions 01, 02 was analyzed by Sephadex G-50M gel filtration chromatography and flight mass spectrograph was employed to analyze the fraction O3. The results showed immobilized papain could depolymerize chitosan successfully, and separating the product by ultrafiltration membrane was feasible. Further study revealed that the immobilized papain could be repeatedly used at least 10 times. An amount of 1 mg free papain and mmobilized papain could produce 2.57 and 17.34 mg chitosan oligomers, respectively. The results indicated that the depolymerization efficiency of immobilized papain was obviously higher than that of free papain.
Chitosan oligomer, Preparation, Immobilized
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林洪, Chang-Hu Xue, Yu Fang, Hong Lin, Lei Chen, Zhao-Jie Li, Deng Deng & Chong-Xiao Lu
Journal of Applied Phycology 13:67-70, 2001.,-0001,():
-1年11月30日
A low molecular weight sulfated polysaccharide (L-A) was prepared by mild acid hydrolysis of crude fucoidan (F-A) from Laminaria japonica. In comparison with F-A, L-A had a lower proportion of fucose residues, but a similar proportion of sulfate. The galactose content of both fractions was relatively high, especially for L-A (57%). The antioxidant properties of the two polysaccharides were studied using two low-density lipoprotein oxidation systems. L-A had a stronger effect against low-density lipoprotein oxidation in both systems. F-A inhibited the AAPH-induced low-density lipoprotein oxidation, but had little effect on the CuCC-induced system due to its large molecular mass. The active sulfated fraction L-A, containing alactose, mannose and fucose (about 9: 2: 2) is reported here for the first time.
antioxidant,, chemical characters,, fucoidan,, Laminaria japonica,, LDL,, low-density lipoprotein
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林洪, 江洁, 许加超, 赵雪, 薛长湖, 李兆杰
营养学报,2002,24(1):87~89,-0001,():
-1年11月30日
贻贝, 大豆, 卵黄, 磷脂, 不饱和脂肪酸
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