从生物化学和物理学两个方面比较了养殖对虾和海捕对虾肌动球蛋白的特性，研究了加热对蛋白的影响。结果表明：加热后两种对虾蛋白质的溶解度和Ca2+2A TPase活性随温度的升高而下降，Mg2+-ATPase活性的变化与Ca2+2A TPase的稍有不同。总巯基数从40℃开始略有下降，未结合巯基数先升后降。疏水性随温度升高而增大。ATP感度在常温时几乎没有变化，而35℃后有明显下降。所有指标的比较均说明，对虾肌动球蛋白对热反应强烈的温度始于30～40℃，且养殖对虾的热稳定性更差。

The phospholipids of mussels (Mytilus edulisl Linne) from the coast of Qingdao were extracted, fractionated and analysed over a 12 month period. Thecontents of total lipids, neutral lipids, polar lipids and phospholipids were meausred. The composition of phosholipids was determined by high-performance liquid chromatiorgraphy, and their fatty acid composition was analysed by gas chromatorgraphy. Thephospholipide contert ranged from 3.6 to 6.4 g kg-1 soft tissue. Pe (phosphatidyl ethanlaminc) and PC (phosphatidyl chline) were the major constituents. C16:0, C20:5 and C22:6 wree the major fatty acids. C20:5 (5.25-23.10%) and C22:6 (6.05-20.42%) varied regularyly with the seasonal factors, Their total amounts were high from January to Juhne, which wouldbe an optimal time for the utilisation of trhe phospholipids of mussels.

Muscle residue depletion of chloramphenicol (CAP), sulphamethoxazole (SMZ) and oxytetracycline (OTC) following oral administration was evaluated in shrimp, Penaeus chinensis under field conditions. Three groups of shrimps were cultured in tanks filled with seawater and fed a commercial medicated diet containing 2000 mg kg 1 CAP, SMZ and OTC, respectively, twice daily for 3 days. Sampling was conducted at different intervals (0, 0.5, 1, 1.5, 2, 3, 4, 8, 10, 12, 16, 24, 36, 48, 72, 96, 120, 144, 168 h) after the cessation of medication. Drug analysis was carried out by HPLC. The elimination half-life (t1/2) of CAP was 10.04 h, the elimination half-life (t1/2) of SMZ was 5.68 h and that of OTC was 16.12 h. If the EU MRPL value of 0.3 ng/g for CAP and MRL value of 0.1 Ag/g for SMZ and OTC quoted for muscle from finfish is extended to shrimp muscle, extrapolation of the data indicates that it would be passed after a 139.7 h (95% CI=132.0-144.4 h), 30.6 h (95% CI=27.2-33.1h), 90.3 h (95% CI=87.9-92.5h) withdrawal period for CAP, SMZ and OTC in shrimp muscle, respectively.

Immobilized papain was used to prepare chitosan oligomers and the optimum conditions were as follows: NaAc-HAc buffer system, pH 4.0, temperature 45℃. After 24 h reaction with stirring, nanofiltration and ultrafiltration were used to desalt and isolate chitosan oligomers into different molecular weight fractions respectively. The yield of chitosan oligomers with molecular weight under 10,000 was 49.55% to total substrate, while that of between 600 and 2000 was 11.07%. The fractions 01, 02 was analyzed by Sephadex G-50M gel filtration chromatography and flight mass spectrograph was employed to analyze the fraction O3. The results showed immobilized papain could depolymerize chitosan successfully, and separating the product by ultrafiltration membrane was feasible. Further study revealed that the immobilized papain could be repeatedly used at least 10 times. An amount of 1 mg free papain and mmobilized papain could produce 2.57 and 17.34 mg chitosan oligomers, respectively. The results indicated that the depolymerization efficiency of immobilized papain was obviously higher than that of free papain.

A low molecular weight sulfated polysaccharide (L-A) was prepared by mild acid hydrolysis of crude fucoidan (F-A) from Laminaria japonica. In comparison with F-A, L-A had a lower proportion of fucose residues, but a similar proportion of sulfate. The galactose content of both fractions was relatively high, especially for L-A (57%). The antioxidant properties of the two polysaccharides were studied using two low-density lipoprotein oxidation systems. L-A had a stronger effect against low-density lipoprotein oxidation in both systems. F-A inhibited the AAPH-induced low-density lipoprotein oxidation, but had little effect on the CuCC-induced system due to its large molecular mass. The active sulfated fraction L-A, containing alactose, mannose and fucose (about 9: 2: 2) is reported here for the first time.