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【期刊论文】Role of Genomic Rearrangements in Producing New Ribotypes of Salmonella typhi
刘树林, IVY NG, SHU-LIN LIU, ? AND KENNETH E. SANDERSON*
JOURNAL OF BACTERIOLOGY, June 1999, p.3536-3541,-0001,():
-1年11月30日
Salmonella typhi is the only species of Salmonella which grows exclusively in humans, in whom it causes enteric typhoid fever. Strains of S. typhi show very little variation in electrophoretic types, restriction fragment length polymorphisms, cell envelope proteins, and intervening sequences, but the same strains are very heterogeneous for ribotypes which are detected with the restriction endonuclease PstI. In addition, the genome of S. typhi has been proven to undergo genomic rearrangement due to homologous recombination between the seven copies of rrn genes. The relationship between ribotype heterogeneity and genomic rearrangement was investigated. Strains of S. typhi which belong to 23 different genome types were analyzed by ribotyping. A limited number of ribotypes were found within the same genome type group; e.g., most strains of genome type 3 belonged to only two different ribotypes, which result from recombination between rrnH and rrnG operons. Different genome type groups normally have different ribotypes. The size and identity of the PstI fragment containing each of the seven different rrn operons from S. typhi Ty2 were determined, and from these data, one can infer how genomic rearrangement forms new ribotypes. It is postulated that genomic rearrangement, rather than mutation, is largely responsible for producing the ribotype heterogeneity in S. typhi.
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刘树林, Shu-Lin Liu, Kenneth E. Sanderson *
FEMS Microbiology Letters 164 (1998) 275-281,-0001,():
-1年11月30日
Partial digestion with I-CeuI, which digests bacterial DNA at the gene coding for the large subunit rRNA, established the rrn genomic skeleton (the distance in kb between rRNA operons) in 56 strains of Salmonella, from Salmonella Reference B (SARB) set. All had seven I-CeuI sites, indicating seven rrn operons. The order of I-CeuI fragments was ABCDEFG in S. typhimurium LT2 and in 31 other species, mostly host-generalists; in S. typhi, S. paratyphi C, S. gallinarum, and S. pullorum (host-specialized species), these fragments are rearranged, due to homologous recombination between the rrn operons. Rearrangements, such as inversions and translocations not involving the rrn operons, are rare. I-CeuI fragments of some species are larger than the norm, suggesting the insertion of unique blocks of DNA by lateral transfer from other species.
Salmonella, Chromosome, I-CeuI, Pulsed-field gel electrophoresis
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【期刊论文】Genomic Cleavage Map of Salmonella typhi Ty2
刘树林, SHU-LIN LIU AND KENNETH E. SANDERSON*
JOURNAL OF BACTERIOLOGY, Sept. 1995, p.5099-5107,-0001,():
-1年11月30日
The genomic cleavage map of Salmonella typhi Ty2, 4,780 kb in size, was determined through digestion of the genomic DNA with endonucleases and separation of the fragments by pulsed-field gel electrophoresis. The chromosome has 33, 26, 7, and 35 sites for the enzymes XbaI, BlnI, I-CeuI, and SpeI, respectively. The fragments were arranged around the chromosome through excision of fragments from the gel, redigestion with a second enzyme, end labelling with 32P, and reelectrophoresis and named in alphabetical order. Tn10 transposons inserted in 82 different genes of Salmonella typhimurium were transduced by phage P22 into S. typhi, and the location of Tn10, and thus of the gene, was mapped through the XbaI and BlnI sites of Tn10. All seven I-CeuI sites (in rrl genes for 23S rRNA) were conserved, and the gene order within the I-CeuI fragments resembles that of S. typhimurium LT2, but the order of I-CeuI fragments is rearranged from ABCDEFG in S. typhimurium LT2 to AGCEFDB in S. typhi. In addition, there is a 500-kb inversion which covers the terminus region. Comparisons of lengths of segments between genes showed that S. typhi has segments which differ in size from those in S. typhimurium. The viaB locus, for synthesis of the Vi antigen of S. typhi, was shown to be within a 118-kb loop (a segment of DNA with no homolog in most other Salmonella species) between mel and poxA on the chromosome.
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【期刊论文】I-CeuI Reveals Conservation of the Genome of Independent Strains of Salmonella typhimurium
刘树林, SHU-LIN LIU AND KENNETH E. SANDERSON*
JOURNAL OF BACTERIOLOGY, June 1995, p.3355-3357,-0001,():
-1年11月30日
The enzyme I-CeuI, encoded by a class I mobile intron inserted in the gene for 23S rRNA in Chlamydomonas eugamatos, cleaves a specific 19-bp sequence in this gene. This sequence is present only in the seven genes for rRNA in Salmonella typhimurium and Escherichia coli. Partial digestion with I-CeuI of DNA from 17 wild-type strains of S. typhimurium indicates that the chromosome of these strains is strongly conserved, for the digestion products closely resemble those of strain LT2. The lengths and order of chromosomal segments are conserved in 15 of the strains; 2 show some rearrangements. XbaI digestion indicated heterogeneity without revealing the genomic structure. Because of conservation of I-CeuI sites in genes for rRNA and conservation of the number and locations of these genes, I-CeuI provides an excellent tool for the rapid examination of the chromosomes of related species of bacteria; differences in the fingerprints indicate the occurrence of chromosomal rearrangements such as insertions or inversions.
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刘树林, 郑君芳①, 刘桂荣①②, 朱万孚①, 周宇光③, 刘树林①②**
中国科学,2003,33(6):514~524,-0001,():
-1年11月30日
阐明根瘤菌之间的系统发育关系,可使人们更有效地利用这些自然资源,因而对农业生产和环境保护具有重大意义。通过-Ceu 酶切表明64株根瘤菌的基因组结构特征,揭示根瘤菌的系统发育关系。结果表明,64株根瘤菌依据基因组结构特征可聚为21个系统发育群,这些群与16SrRNA分子聚群结果大致相符,但与现行的根瘤菌分类体系所得结果有较大差异。
根瘤菌, 系统发育, 基因组结构
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