提示
恭喜！关注成功
期刊论文已为您找到该学者16条结果 成果回收站
上传时间
2005年01月25日
苏晓东， Hui Ren， a， Yuhe Liang， b， Rui Li， Haitao Ding a， Shihong Qiu， c， Shanyun Lua， Jianli An a， Lanfen Li a， Ming Luo， c Xiaofeng Zheng a， * and Xiao-Dong Sua
Acta Cryst. (2004). D60, 1292-1294，-0001，（）：
-1年11月30日
The adrenal gland protein AD-004 was identified in the human adrenal gland. Full-length AD-004 contains 172 amino acids, with a predicted molecular weight of about 20kDa. In attempts to crystallize human AD-004, the gene was subcloned into a modified pET vector, pET21-DEST, with an N-terminal His5 tag using the Gateway cloning system, followed by protein expression in Escherichia coli strain BL21(DE3). The protein was purified in two steps to near-homogeneity and was crystallized. The crystals belong to space group P61 or P65, with unit-cell parameters a= b=99.56, c= 57.19 A
-
46浏览
-
0点赞
-
0收藏
-
0分享
-
101下载
-
0
-
引用
>
