您当前所在位置: 首页 > 学者
在线提示

恭喜!关注成功

在线提示

确认取消关注该学者?

邀请同行关闭

只需输入对方姓名和电子邮箱,就可以邀请你的同行加入中国科技论文在线。

真实姓名:

电子邮件:

尊敬的

我诚挚的邀请你加入中国科技论文在线,点击

链接,进入网站进行注册。

添加个性化留言

已为您找到该学者10条结果 成果回收站

上传时间

2005年02月24日

【期刊论文】Evolution and expression of the amphioxus AmphiHMGB gene encoding an HMG-box protein

张士璀, Zhenhui Liu a, Shicui Zhang a, b, *, Mei Liu a, Yongjun Wang a, Jiansong Chu a, Anlong Xu c

Comparative Biochemistry and Physiology Part B 137(2004)131-138,-0001,():

-1年11月30日

摘要

Amphioxus HMGB gene, AmphiHMGB, encoding a high mobility group (HMG) protein was identified from the gut cDNA library of Branchiostoma belcheri tsingtauense. Primary structural examination indicates that it is a member of HMGB family. Molecular phylogenetic analysis suggests that AmphiHMGB represents the archetype of chordate HMGB genes including HMGB1, HMGB2 and HMGB3 lthough it may have lost its C-terminal domain during evolution. Northern blotting revealed that AmphiHMGB expression was restricted mainly to guts in the adult, and AmphiHMGB transcripts were in greater abundance in gastrula and neurula though they were markedly low in 1-day larva.

Amphioxus, Branchiostoma, HMGB, Phylogenetics, Evolution, Northern blot, Expression, Archetype

上传时间

2005年02月24日

【期刊论文】Presence and Characterization of Complement-like Activity in the Amphioxus Branchiostoma belcheri tsingtauense

张士璀, Shicui Zhang*, Changfa Wang, Yongjun Wang, Ran Wei, Guohu Jiang and Hong Ju

ZOOLOGICAL SCIENCE 2003: 1207-1214 (2003),-0001,():

-1年11月30日

摘要

The humoral fluid of Branchiostoma belcheri tsingtauense was examined for the presence of complement-like activity. The humoral fluid showed hemolytic activity for rabbit erythrocytes and those from species representing mammals, birds, amphibians and fish, but not sensitized sheep rythrocytes. There was no relationship between phylogeny of the target erythrocytes and degree of hemolysis. The hemolytic activity was optimally assayed at 20℃, at pH 7.5, and in the presence of 10mM Mg2+. The hemolytic activity was Mg2+ -dependent and heat-sensitive, and was abrogated by treatment with rabbit anti-human C3 serum, zymosan, methylamine, hydrazine, and henylmethylenesulfonyl fluoride. In addition, Western blotting and titration by turbidimetric immunoassay (TIA) revealed that amphioxus humoral fluid contained C3 component, and its oncentration is about 1.17mg/ml, which is comparable to C3 concentration in human or dog sera. These suggest that the hemolytic activities displayed by amphioxus humoral fluid appear to represent the vertebrate complement system probably operating via the alternative pathway.

amphioxus,, humoral fluid,, hemolytic activity,, alternative pathway (, AP), ,, complement 3 (, C3),

上传时间

2005年02月24日

【期刊论文】C-banding pattern and nucleolar organizer regions of amphioxus Branchiostoma belcheri tsingtauense Tchang et Koo, 1936

张士璀, Shicui Zhang, Changliu Wang, & Jiansong Chu

Genetica 121: 101-105, 2004.,-0001,():

-1年11月30日

摘要

Chromosome banding study remains lacking in amphioxus heretofore. This paper reports the C-band pattern of the metaphase chromosomes of amphioxus Branchiostoma belcheri tsingtauense, the first description of banded karyotype in cephalochordate. The C-banding showed that around 50% of the second chromosome pair examined was heteromorphic, while the remaining ones were homomorphic. In contrast, the other 17 pairs were all homomorphic. These broadly support our previous suggestion that the second chromosome pair is a pair of sex-chromosomes. The C-banding stained positively about 54.3% of the chromosome surface. Most of the centromeric and terminal regions in many chromosomes had positive C-bands, and some interstitial C-bands were also observed in some chromosomes. It seems clear that chromosomes of amphioxus are highly heterochromatinized. In addition, silver staining revealed a single pair of nucleolar organizer regions (NORs) located in the telomeric regions of mediumsized chromosomes. It is suggested that the number and location of NORs in amphioxus represent the primitive condition for chordates.

amphioxus,, Branchiostoma,, C-banding,, NORs

上传时间

2005年02月24日

【期刊论文】Cloning and phylogenetic analysis of an amphioxus myogenic bHLH gene AmphiMDF

张士璀, Jinduo Yuan, a Shicui Zhang, a, * Zhenhui Liu, a Zhidong Luan, b and Gengxi Hu b

Biochemical and Biophysical Research Communications 301(2003)960-967,-0001,():

-1年11月30日

摘要

In thiss tudy, a member of the MyoD gene family, AmphiMDF, wasis olated from the embryos of amphioxusby degenerate PCR, followed by rapid amplification of cDNA ends (RACE). Southern blot analysis confirmed that only a single myogenic bHLH gene waspres ent in the genome of amphioxus Branchiostoma belcheri tsingtauense. Sequence and phylogenetic analyses indicated that AmphiMDF falls at the base of its vertebrate homologs. The amino acid sequence of AmphiMDF was almost equally similar to those of the four clusters of the vertebrate MyoD family. This suggests that AmphiMDF is not only the sister but also the archetype of the vertebrate myogenic bHLH genes. The scenarios to explain the origin of the vertebrate MyoD gene family from the ancestral myogenic bHLH gene like AmphiMDF are also discussed.

Amphioxus, Branchiostoma belcheri tsingtauense, Archetype, Myogenic bHLH gene, Evolution, Phylogeny

上传时间

2005年02月24日

【期刊论文】Phenoloxidase, a marker enzyme for differentiation of the neural ectoderm and the epidermal ectoderm during embryonic development of amphioxus Branchiostoma belcheri tsingtaunese

张士璀, Guorong Li a, Shicui Zhang b, *, Jianhai Xiang a

Mechanisms of Development 96(2000)107-109,-0001,():

-1年11月30日

摘要

The development of phenoloxidase during amphioxus embryogenesis was spectrophotometrically and histochemically studied for the first time in the present study. It was found that (1) PO activity initially appeared in the general ectoderm including the neural ectoderm and the epidermal ectoderm at the early neurula stage but not in the mesoderm or the endoderm, and (2) PO activity disappeared in the neural plate cells but remained unchanged in the epidermal cells when the neural plate was morphologically quite distinct from the rest of the ectoderm. It is apparent that PO could serve as a marker enzyme for differentiation of the neural ectoderm from the epidermal ectoderm during embryonic development of amphioxus.

Phenoloxidase, Amphioxus, Nervous system, Differentiation

合作学者

  • 张士璀 邀请

    中国海洋大学,山东

    尚未开通主页