Selective plasma treatment of the recessed regions of the elastomer stamps is shown to alter the resultant protein patterns. Fluorescence microscopy is demonstrated to be an excellent tool to discriminate between regions of microcontact printed fluorescent dye-labelled albumin in polystyrene. Atomic force microscopy and shear force microscopy are used to provide high-resolution images of the patterned protein layers. The formation and characteristics of the patterns formed by these alternative strategies is discussed.

设计并制备了一种新的具有分子探针功能的主体分子型非病毒载体-邻菲啰啉-β-环糊精衍生物主体分子（DZY-1），应用凝胶电泳法研究探讨了DZY-1与DNA相互作用及客体分子对该主体分子诱导DNA凝聚的影响；并进一步研究探讨了单一主体分子、主/客体分子配合物诱导DNA分子凝聚和聚合所形成的超分子聚合物抗限制性内切酶（HindⅢ）酶解的特性. 应用扫描电子显微镜（SEM）观测到该主体分子、主/客体分子配合物与DNA分子相互作用形成超分子聚合物的微观结构形态。阐述了其作为非病毒基因载体可能的传递机制，为设计、制备新的非病毒基因载体提供了一种新途径。

Rhodamine B-ethylenediamine-cyclodextrins (RhB-βCDen) and rhodamine B-cyclodextrins (RhB-βCD) can form inclusive complexes with many guest molecules, a reaction which can be used as a nucleic acid probe. In this paper, the most stable conformations of RhB-βCDen and RhB-βCD have been determined by fluorescence experiments and analyzed by molecular modeling simulation. The interaction between RhB-βCDen and two guest molecules, 1-borneol and cyclohexanol, has also been investigated. The results showed that RhB-βCDen has a stronger interaction with 1-borneol than with cyclohexanol. Borneol could push the three aromatic-rings of rhodamine B out of the CD cavity, while the cyclohexanol could not. The interactive sites of host andguest are also presented.

Condensation of DNA by multivalent cations can provide useful insights into the physical factors governing the folding and packaging of DNA in vivo. In this work, local ordered structures of spermidine-DNA complexes prepared from different DNA concentrations have been examined by using atomic force microscopy (AFM) and polarizing microscopy (PM). Two types (I and II) of DNA condensates, significantly different in sizes, were observed. It was found that for extremely dilute solutions (DNA concentrations around 1ng/ml or below), the DNA molecules would collapse into toroidal structures with a volume equivalent to a single l-DNA (type I). In relatively dilute solutions (DNA concentrations between 1 and 10ng/ml), a significantly larger structure of multimolecular toroids (circular and elliptical, type II) were formed, which were constructed by many fine particles. Measurements show that the average diameter of these fine particles was similar to the outer diameter of the monomolecular toroids observed in extremely dilute solutions, and the thickness of the multimolecular toroids had a distribution of multi-layers with height increments of 11 nm, indicating that the multimolecular toroidal structures have lamellar characteristics. Moreover, by enriching the DNA-spermidine complexes in very diluted solution, branch-like structures constructed by subunits were observed by using AFM. The analysis of the pellets in polarizing microscopy reveals a liquid-crystal-like pattern. These observations suggest that DNA-spermidine condensation could have multiple stages, which are very sensitive to the DNA and spermidine concentrations.

The microcontact printing (CP) technology available for patterning protein, DAN hybridixation, immu-nossay and cellures onto solid surface are reviewed. This review describes some of the techniques currently employed for creating two-dimensional biomolcular microarry, and the research results regarding also presented.