Received 26 January 1998; received in revised form 19 May 1998; accepted 20 May 1998 The results from the measurement of the fluorescence spectrum showing the binding characteristics of acridine orange (AO) and bovine serum albumin (BSA) are reported. It was found that the equilibrium constant k-4848.64 lmol1, and the number of binding sites n-0.82. Based on the mechanism of the Forster energy transference, the transfer efficiency of energy and distance between the acceptor AO and BSA were found. The interaction between AO and BSA have been verified as consistent with the static quenching procedure and the quenching mechanism is related to the energy transfer.

Microcontact printing (μCP) is aneasy andefficientway of producing patterns of self-assembledmonolayers (SAM) containing different functional groups. We have developed a simple and convenient μCP-based technique for the modification of a mica substrate with 3-aminopropyltriethoxysilane (APTES) and the micropatterning of proteins (chicken IgG and rabbit IgG) on the modified mica surface. Our approach provides a quick and easy way to produce protein patterns on solid surfaces. The printed immunoglobulin patterns were detected by exposing the substrate to solutions containing fluorescently labeled complementary anti-IgGs, and the formed immunoassays were studied using fluorescence microscopy. Copyright 2005 John Wiley & Sons, Ltd.

The microcontact printing (CP) technology available for patterning protein, DAN hybridixation, immu-nossay and cellures onto solid surface are reviewed. This review describes some of the techniques currently employed for creating two-dimensional biomolcular microarry, and the research results regarding also presented.