利用桥接物修复1.0cm以上神经缺损，效果不理想。本实验采用自体静脉桥接家兔4.0cm长胫神经缺损，术后2个月未发生神经再生现象。而利用自体静脉内种植雪旺氏细胞匀浆，桥接家兔4.0cm长胫神经缺损，术后电生理、组织学、透射电镜等项检测指标都能观察到神经再生现象。雪旺氏细胞匀浆在静脉桥接物的这一作用，增加了非神经移植物修复神经缺损的长度。

observed it s activity in stimulating neurite outgrowth in vitro. Methods cDNA encoding mature human HBNF was amplified from total RNA isolated from an 182week aborted human fetal brain by RT-PCR method. After amplification, the HBNF cDNA gene was cloned into p PIC9K, a shuttle expression vector for yea stsystem. The positive clone of expression vector bearing HBNF cDNA gene was obtained by screening. Verified recombinant vector was then used to transform Pichiastrain GS115 by electroporation. His+ transformants were selected on minimal dextrose medium (MD) plates which were histidine free1 His+ yeast recombinants with multi-copy insert s were screened in vivo by their resistance to G4181 PCR analysis was used to confirm the integration of the HBNF cDNA gene into the Pichia genome1 Secreted expre ssion of hrHBNF protein in culture medium was obtained when the positive clone containing the HBNF cDNA gene was induced by methanol. The hrHBNF product purified by gel chromatography was added to cultured rat pheochromocytoma (PC12) cells to observe it s ability to stimulate neurite outgrowth. Results In the recombinant expression vector, the insert was sequenced to show exactly the sequence encoding human HBNF according to Genbank data. The HBNF cDNA gene was cloned downstream to the α-factor, and it s open reading frame was in frame with the α-factor signal sequence in p PIC9K1 SDS2PAGE showed that the molecular weight of the induced expression product was about 18kDa, consistent with that of human HBNF reported in the literature. The protein product did promote neurite outgrowth in cultured rat pheochromocytoma (PC12) cells. Conclusion Recombinant human heparin-binding neurite-promoting factor can be expressed with a yeast system, and it s product possesses the biological activity to promote neurite outgrowth.

Objective: To treat the loss of part of the forearm with a multi-dimension-freedom electronic artificial hand, which is controlled by a reconstructed finger transplanted from the second toe to the forearm stump. Methods: The female patient was 19 years old, whose right hand and wrist were crushed into pieces by machine at work and her forearm was amputated at the level of 8cm proximal to the wrist. The second toe of her left foot was transplanted to reconstruct the digit onto the stump of her forearm. Two months after the transplantation, the patient was transferred to the rehabilitation center for further rehabilitation training, which consisted of: training for adaptation to weight bearing, testing and training of sensibility to weight. testing and training for stability of the hand, and testing and training for the controlling function of the reconstructed digit. Results: The transplanted toe survived well. After rehabilitation the reconstructed digit functioned well. In testing the performance under control mandate, the accuracy rate of the electronic artificial hand was 100%. Conclusions: A 100% accuracy rate of the electronic artificial hand can be achieved by transplantation of the toe onto the stump of the forearm. It provides a useful pathway and an example for improvement of control accuracy of a multiple-freedom electronic artificial hand and reduction of false action.

It was un satisfactory to use bridging material to repair nerve defect longer than 1.0cm. This experiment used autogenous vein to bridge rabbit tibia nerve with 4.0cm defect. The nerve regeneration did not occur two months after the operation. But when using autogenous vein bridging implanted with Schwann cell homogenate, the electrophysiology, histology, transmission electron microscopy, etc. indicated that the nerve regeneration did occur. This result showed that the length of reparation of nerve defect using non-nerve graft might be increased.