observed it s activity in stimulating neurite outgrowth in vitro. Methods cDNA encoding mature human HBNF was amplified from total RNA isolated from an 182week aborted human fetal brain by RT-PCR method. After amplification, the HBNF cDNA gene was cloned into p PIC9K, a shuttle expression vector for yea stsystem. The positive clone of expression vector bearing HBNF cDNA gene was obtained by screening. Verified recombinant vector was then used to transform Pichiastrain GS115 by electroporation. His+ transformants were selected on minimal dextrose medium (MD) plates which were histidine free1 His+ yeast recombinants with multi-copy insert s were screened in vivo by their resistance to G4181 PCR analysis was used to confirm the integration of the HBNF cDNA gene into the Pichia genome1 Secreted expre ssion of hrHBNF protein in culture medium was obtained when the positive clone containing the HBNF cDNA gene was induced by methanol. The hrHBNF product purified by gel chromatography was added to cultured rat pheochromocytoma (PC12) cells to observe it s ability to stimulate neurite outgrowth. Results In the recombinant expression vector, the insert was sequenced to show exactly the sequence encoding human HBNF according to Genbank data. The HBNF cDNA gene was cloned downstream to the α-factor, and it s open reading frame was in frame with the α-factor signal sequence in p PIC9K1 SDS2PAGE showed that the molecular weight of the induced expression product was about 18kDa, consistent with that of human HBNF reported in the literature. The protein product did promote neurite outgrowth in cultured rat pheochromocytoma (PC12) cells. Conclusion Recombinant human heparin-binding neurite-promoting factor can be expressed with a yeast system, and it s product possesses the biological activity to promote neurite outgrowth.

应用带血管腓骨治疗上肢侵袭性良性骨肿瘤和恶性骨肿瘤10例，不带血管腓骨治疗桡骨远端骨巨细胞瘤13例，总共23例。随访时间2～12年，随访中使用ECT（单光子照射扫描照相），彩色多普勒血管超声检查，以及X线片检查。结果显示带血管腓骨10例移植骨片生长良好，移植骨片与受骨愈合牢固；不带血管腓骨移植骨片长度超过6cm时愈合困难。本组带血管腓骨移植骨片最长26cm，最短9cm，这种手术最大的进步是病变组织可彻底切除，切除后可一期重建缺损组织。另一个优点是移植后的骨片周径可有增粗。

Objective To compare the effect of vascularized and non-vascularized fibula grafting performed for the reconstruction of bone defects after the resection of bone tumors. Methods Vascularized fibula grafting was performed in 18 patients with malignant or aggressive benign bone tumor of the extremities. Non-vascularized fibula grafting was performed in 15 patients with giant cell tumor of the radius. All the 18 patients were followed up with radiography, single photon emission computed tomography (SPECT), colored Doppler's sonography (CDS) examinations and functional observation for 2 to 14 years. Results Vascularized fibula grafts with bone defects of 9 to 26cm showed good blood supply in emission computerized tomography (ECT) and radiography examinations, and bone union was achieved (Table 1). In non-vascularized fibula grafting cases (Table 2), bone union was not achieved. Cconclusions The advantages of vascularized grafting are the feasibility of one-stage reconstruction of various tissues and the growth of grafted fibula. It is necessary to adopt vascularized fibula grafting to reconstruct large bone defect resulting from the resection of extensive bone tumors, such as giant cell tumor (GCT), fibrous dysplasia of bone and malignant bone tumors.

利用桥接物修复1.0cm以上神经缺损，效果不理想。本实验采用自体静脉桥接家兔4.0cm长胫神经缺损，术后2个月未发生神经再生现象。而利用自体静脉内种植雪旺氏细胞匀浆，桥接家兔4.0cm长胫神经缺损，术后电生理、组织学、透射电镜等项检测指标都能观察到神经再生现象。雪旺氏细胞匀浆在静脉桥接物的这一作用，增加了非神经移植物修复神经缺损的长度。