观察不同参数（频率、脉宽、峰值）组合的陡脉冲电场对恶性肿瘤细胞的杀伤效应。将癌细胞悬液3ml盛于玻璃瓶中，每次施加不同参数组合的电脉冲20min，用MTT法测细胞死亡率，并制作生长曲线；观察细胞的超微结构变化。结果发现：不同参数组合的陡脉冲对癌细胞的杀伤有较大影响，随着参数值的增大，细胞死亡率上升，细胞的增殖受到抑制；实验组的细胞肿胀，溶解性坏死，仅留细胞轮廓，细胞膜完整性破坏，线料体空化、肿胀。

为输电线路的抗冰设计和防冰技术措施以及在实验室中模拟导线覆冰提供理论依据，根据导线覆冰的物理过程对导线覆冰过程中的热平衡进行了系统分析，提出了导线覆冰的热平衡方程，并因此得到了导线覆冰的冻结系数、湿增长覆冰向干增长转变的临界条件以及临界条件均为环境温度、风速、水滴直径、空气中液态水含量及导线传输电流时导线覆冰的函数。通过仿真计算，得出了覆冰干湿增长临界条件下和导线覆冰临界条件下环境温度、风速、水滴直径、空气中液态水含量及导线传输电流等参量之间的关系，并提出可以通过提高线路传输电流的方法达到减少或防止导线覆冰的目的。

Electroporation (i.e., reversible electrical breakdown) has been used for biomedicine such as electroporation therapy, transdermal drug delivery, cellular drug carrier systems, and gene transfer therapy. However, little research on the biological effect of irreversible electrical breakdown has been published. In this paper, the killing and inhibiting effects of pulsed electric field, whose rising edge is 100ns level (we call it steep pulsed electric field, SPEF), to target cancer cell and solid tumor were successfully observed. The SPEF consists of two parts: the rising process containing ample high-frequency components, and the falling process containing lots of low-frequency components. Cell exposed to the pulse presented multiple changes: destructed membrane, swollen nucleus, disintegrated karyotheca, and cavitated, swollen mitochondria in histological and ultrastructure observations. SPEF blocked cell transition from G0/G1 phase to S+G2/M phase in flow cytometry analysis, and tumor growth retardation was observed in tumor-bearing mice. These experimental results show SPEF have a certain function to generate irreversible electrical breakdown in tumor cells, and might provide a new promising therapeutic approach for tumor treatment.