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杨洪强, Hong-Qiang Yang, Yu-ling Jie, Lai-Xin Liu, Wen-Yun Tang and Ming-ge Yu
,-0001,():
-1年11月30日
The effects of abscisic acid (ABA) on pollen germination and tube growth were studied in pomegranate (Punica granatum L.). Results showed that ABA inhibited pollen germination and tube growth at higher concentrations, over 50 mM, while low ABA concentrations, 0.05~5 mM, showed lower inhibition. The percentage of pollen germination did not change, but both tube growth and the effects of ABA on tube growth were inhibited by 10 mg·L-1 cycloheximide treatment. The inhibitors of ABA biosynthesis, NDGA (nordihydroguaiaretic acid) and fluridone, inhibited pollen germination and tube growth evidently. The degree of inhibition increased gradually along with the increases in ABA concentrations from 20 to 100 mM, and the inhibitory response to tube growth was more significant. ABA (1 mM) could abate the inhibition degree of NDGA and fluridone to tube growth, but could not influence the germination. The result indicated that low ABA concentration was necessary for normal pollen growth.
Punica granatum, pollen germination, pollen tube growth, ABA, fluridone, nordihydroguaiaretic acid, cycloheximide
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