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【期刊论文】Chemiluminescence of luminol catalyzed by silver nanoparticles
崔大祥, Hao Chen, Feng Gao∗, Rong He, Daxiang Cui∗
Journal of Colloid and Interface Science 315(2007)158-163,-0001,():
-1年11月30日
Silver nanoparticles (AgNPs) are synthesized by chemical reduction method and characterized by UV-vis spectra, transmission electron microscopy, and high performance particle sizer.We have found that AgNPs could enhance the chemiluminescence (CL) intensity of luminol-H2O2 system. In this reaction, luminol intermediate is generated under alkaline condition on the surface of AgNPs in luminol-H2O2 system and enhances CL intensity. To validate the reaction mechanism, AgNPs are bound with thioglycolic acid (Ag-HSCH2COOH) and then joined to BSA protein (Ag-BSA). We investigate the CL intensity in the presence of Ag-HSCH2COOH or Ag-BSA comparing with that in the presence of AgNPs and conclude the catalytic reaction take place on the surface of AgNPs.
Silver nanoparticles, Chemiluminescence, Luminol, Mechanism
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【期刊论文】Characterization of BRCAA1 and Its Novel Antigen Epitope Identification
崔大祥, Daxiang Cui, , Guiqiu Jin, Tianwen Gao, Tianbai Sun, Furong Tian, Giovani Gomez Estrada, Huajian Gao, and Akinori Sarai
Cancer Epidemiol Biomarkers Prev 2004, 13 (7),-0001,():
-1年11月30日
Looking for novel breast cancer antigen epitopes is helpful for its treatment, diagnosis, and prevention. brcaa1 gene is mapped at 1q42.1-q43, its whole genome is 93.857 kb, including 18 exons and 17 introns. BRCAA1 protein is composed of 1,214 amino acids with 10 glycosylate sites, and shares 37% amino acid identity and an identical antigen epitope with Rb binding protein 1. The novel antigen epitope, SSKKQKRSHK, was predicted to locate in the region 610 to 619 sites, was synthesized, and its antibody was fabricated. Competent inhibition analysis showed that SSKKQKRSHK is the shortest effective peptide. The antigen epitope was mapped in the cytoplasm of MCF-7 cells. Immunohistochemistry analysis showed that the antigen epitope exhibited positive expression in 65% (39 of 60) breast cancer specimens and negative expression in 60 noncancerous tissues. Statistical analysis shows that its expression is closely associated with status of ER and PR, with sensitivity of 100% and specificity of 81%, and confidence interval of 85.9% to 96.9%. ELISA analysis showed that the mean absorbance of sera antibody titers from breast cancer patients and healthy donors were 0401 F 0.163 SD and 0.137 F 0.121 SD, respectively. Sixty-four percent breast cancer patient sera and 13% healthy donor sera had higher titer than mean titer of healthy donors, and there exists significant difference between breast cancer patients and healthy donors (P<0.001). In this study, a novel breast cancer antigen epitope, SSKKQKRSHK, is identified. Its expression is associated with characteristics that are themselves associated with prognosis of breast cancer, and its sera antibody level may be helpful for breast cancer diagnosis.
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【期刊论文】A microarray-based gastric carcinoma prewarning system
崔大祥, Da-Xiang Cui, Li Zhang, Xiao-Jun Yan, Ling-Xia Zhang, Jun-Rong Xu, Yan-Hai Guo, Gui-Qiu Jin, Giovani Gomez, Ding Li, Jin-Rong Zhao, Fen-Chan Han, Ju Zhang, Jia-Le Hu, Dai-Ming Fan, Hua-Jian Gao
World J Gastroenterol 2005, 11 (9): 1273-1282,-0001,():
-1年11月30日
To develop a microarray-based prewarning system consisting of gastric cancer chip, prewarning data and analysis software for early detection of gastric cancer and pre-cancerous lesions.
Microarray, Prewarning, Gastric cancer
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