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秦浚川, Haiqin Lu, Jie Zhu, Yuhui Zang, Yuguan Ze, Junchuan Qin*
Biochemical Pharmacology 70(2005)1019-1025,-0001,():
-1年11月30日
Human paraoxnase-3 (hPON3) (EC3.1.8.1) is a lipid-associated enzyme with antioxidant activity, and can inhibit the oxidation of lowdensity lipoprotein (LDL), thereby inhibiting early atherogenic process. In the present study, human PON3 gene was cloned from Human Fetal Liver Marathon-Ready cDNA and expressed in insect cells using baculovirus vector. Twenty-eight milligrams of purified recombinant hPON3 (rhPON3) was obtained from 1 L Sf9 cells culture. The Km and Vmax values of rhPON3, with respective to phenylacetate hydrolysis were 7.46
Human paraoxonase-3, Baculovirus-mediated expression system, Sf9 cell, Gene expression, LDL oxidation, Dihydrocoumarin
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秦浚川, QIN JUN-CHUAN† AND ROBERT F. WEAVER*
JOURNAL OF VIROLOGY, July 1982, p. 234-240,-0001,():
-1年11月30日
Viral RNA from fall armyworm (Spodoptera frugiperda) cells infected with Autographa californica nuclear polyhedrosis virus contains cap structures. Most of the cap labeled in vivo with [3H]methionine or 32pI cochromatographed on DEAE-cellulose with the -5 charge marker; a minor com onent appeared at -4 net charge. The former is probably a cap 1 structure (m GpppXmYp), and the latter is probably a cap 0 (m7GpppXp). On the basis of relative labeling of the two caps with [3H]adenosine and [H]guanosine, we concluded that each cap contained at least one adenosine residue in addition to guanosine and, therefore, that cap 0 contained m7GpppAp. Cleavage of [3H]methionine-labeled viral RNA with tobacco acid pyrophosphatase released a labeled component that cochromatographed on polyethyleneimine-cellulose with m7Gp, confirming the m7GpppX linkage in the cap. These results were also seen with host polyadenylated RNA. The caps appeared in nearly equal abundance in viral polyadenylated and nonpolyadenylated RNAs. The ratio of 32p; incorporated into the cap to that incorporated into mononucleotides suggested average lengths for the polyadenylated and non-polyadenylated RNAs of 1,800 and 1,200 nucleotides, respectively.
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