目的：研究乙肝病毒核心抗原（HBcAg）DNA疫苗诱导的细胞免疫应答。方法：用表达乙肝病毒核心抗原N端144个氨基酸的重组质粒DNA（简称pHBc144）100μg免疫C57BL/6小鼠，用细胞内细胞因子染色技术检测小鼠体内抗原特异性CD8+T细胞的动态变化。结果：pHBc144免疫后抗原特异性CD8+细胞逐渐增多，在初次免疫的第14天出现第一个免疫应答高峰，此后抗原特异性CD8+细胞数量逐渐下降进入免疫记忆期并在1年内维持在稳定水平。加强免疫后在第10天抗原特异性CD8+T细胞数量达到高峰，约是初次免疫应答高峰的2倍，此后抗原特异性CD8+T细胞数量逐渐下降，但下降的速度比初次免疫应答减缓。结论：pHBc144DNA疫苗可诱导有效的细胞免疫应答。

SARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID5.0 of the live virus 103.6 to 104.6 times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed.