AIM: To investigate the effect of ethylene diamine tetraacetic acid (EDTA) on proliferation of rat colonic cells. METHODS: EDTA was administered into Wistar rats, carcinogenesis induced by 1,2-dimethylhydrazine (DMH) in rats was studied with immunohistochemistry. RESULTS: Marked regional differences in cell proliferation were found in all groups. In EDTA-treated animals, total labelling indexes in both proximal (10.00

Accumulating evidence demonstrates that resveratrol, a natural polyphenolic compound extracted from plants, inhibit inflammation when administered. It has direct effects on suppression of platelet coagulation and cytokines production in many experimental models. Because microcirculation occlusion and cytokines over-production is involved in many diseases such as acute pancreatitis (AP), the discovery of resveratrol as platelet and cytokines inhibitors has shed light on the treatment of AP, which still has significant mortality and morbidity. It is anticipated that this natural polyphenol could serve as a therapeutic compound in managing AP through different pathways.

目的 通过体外诱导高效特异的抗肝癌细胞系免疫反应，制备一种以树突状细胞（Dc）为基础的肝癌疫苗。方法 分离人外周血单核细胞，经IL-4和GM-CSF联合刺激诱导DC分化。培养第4天，DC用人肝癌细胞系SMMC-7721的冻融抗原致敏。培养第7天，将肝癌冻融抗原致敏的Dc和未经肝癌冻融抗原致敏的DC分别与T淋巴细胞混合培养24h作为效应细胞，以人SMMC-7721肝癌细胞、MCF-7乳腺癌细胞和PC-3前列腺癌细胞作为靶细胞，用MTT法检测细胞毒活性。结果 经肝癌冻融抗原致敏的DC对SMMC7721细胞有高效而特异的杀伤活性，对其他肿瘤细胞系也有一定的杀伤活性，但无明显特异性；未经肝癌冻融抗原致敏的DC对3种肿瘤细胞系均有一定的杀伤活性，但无明显特异性；各组效应细胞对靶细胞的杀伤率随效靶比(EC/TC)的升高而增强。结论 以肝癌细胞冻融抗原冲击致敏的DC刺激T淋巴细胞增殖在体外可诱导产生高效特异性的抗肝癌免疫反应，为肝癌的主动特异性免疫治疗提供了可靠的理论依据。