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梁宋平, Lingyun Huang, Bingxia Li, Chen Luo, Jinyun Xie, Ping Chen, Songping Liang
Proteomics 4(2004)235-243,-0001,():
-1年11月30日
Recently, it was found that in the gynogenetic haploid and diploid embryos of goldfish, which have exactly the same genome, the haploid condition results in obstruction of gene expression and abnormal development while the diploid embryos have normal gene expression and development. A diploid-dependent regulatory apparatus was proposed to regulate gene expression. To study the difference at the protein expression level of the embryos of haploid and diploid in development, we extracted the total proteins of both the gynogenetic haploid and diploid embryos of goldfish in the same eye formation stage. Two-dimensional polyacrylamide gel electrophoresis was used to separate proteins. The stained gel images were analyzed with the PDQUEST software.A part of protein spots that were differentially expressed in haploid and diploid embryos were identified by matrix assisted laser desorption/ionisation-time of flightmass spectrometry and database analysis. Sixteen protein spots that were absolutely different (only expressed in diploid embryos but not in haploid embryos or vice versa)and 16 protein spots that were up- and downregulated were identified unambiguously,which include some proteins that are correlative with eyes development, nerve development,developing regulation, cell differentiation, and signal transduction. The different significantly gene expression during embryos developing between diploid and haploid is demonstrated.
Goldfish embryo, Mass spectrometry, Proteome analysis, Two-dimensional gel electrophoresis
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梁宋平, Peng-Fei Zhang, Ping Chen, Wei-Jun Hu, Song-Ping Liang*
Toxicon 42(2003)15-20,-0001,():
-1年11月30日
two toxins exhibits sequence identity with other spider toxins such as ProTx-I (64%), SGTx (57%), SNX-482 (55%), and Hanatoxin (54%). HWTX-V can reversibly paralyze locusts and cockroaches for several hours with a ED50 value as 16±5mg/g to locusts, and a larger dose of the toxin can cause death. However, mHWTX-V shows no significant effect on locusts and cockroaches. The structure–activity relationship indicates that the residues Phe34 and Ser35 in the C-terminus of HWTX-V are the key residues of the biological activity.c 2003 Elsevier Science Ltd. All rights reserved.
Huwentoxin-V, The mutant of Huwentoxin-V, Key amino acid residues, Insecticidal toxin
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梁宋平, Yu-Cheng Xiao, Song-Ping Liang*
Toxicon 41(2003)643-650,-0001,():
-1年11月30日
A neurotoxic peptide, named Hainantoxin-V (HNTX-V), was isolated from the venom of the Chinese bird spider Selenocosmia hainana. The complete amino acid sequence of HNTX-V has been determined by Edman degradation and found to contain 35 amino acid residues with three disulfide bonds. Under whole-cell patch-clamp mode, HNTX-V was proved to inhibit the tetrodotoxin-sensitive (TTX-S) sodium currents while it had no any effects on tetrodotoxin-resistant (TTX-R) sodium currents on adult rat dorsal root ganglion neurons. The inhibition of TTX-S sodium currents by HNTX-V was tested to be concentrate-dependent with the IC50 value of 42.3nM. It did not affect the activation and inactivation kinetics of currents and did not have the effect on the active threshold of sodium channels and the voltage of peak inward currents. However,100nM HNTX-V caused a 7.7mV hyperpolarizing shift in the voltage midpoint of steady-state sodium channel inactivation.The results indicated that HNTX-V inhibited mammalian voltage-gated sodium channels through a novel mechanism distinct from other spider toxins such as d-ACTXs, m-agatoxins I-VI which bind to receptor site three to slow the inactivation kinetics of sodium currents.
Spider, Sodium current, Whole-cell recording, Dorsal root ganglion
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梁宋平, Dongling Li a, Yucheng Xiao b, Weijun Hu b, Jinyun Xie b, Frank Bosmans c, Jan Tytgat c, Songping Liang b, *
FEBS Letters 555(2003)616-622,-0001,():
-1年11月30日
Hainantoxin-I is a novel peptide toxin, purified from the venom of the Chinese bird spider Selenocosmia hainana(=Ornithoctonus hainana). It includes 33 amino acid residues with a disulfide linkage of I-IV, II-V and III-VI, assigned by partial reduction and sequence analysis. Under two-electrode voltage-clamp conditions, hainantoxin-I can block rNav1.2/B1 and the insect sodium channel para/tipE expressed in Xenopus laevis oocytes with IC50 values of 68
Hainantoxin-i, Neurotoxin, Sodium channel, Solution structure, ICK motif
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