Objective To assess whether interleukin-10 (IL-10) is chondropotective in vitro. Methods Chonrocytes Ⅱ. The first passage cells were grown in 24-well plates with DMEM, supplemented with 10% fetal bovine serum, for 2-4 days. The cells were then cultured in 0.1% fetal bovine serum DMEM medium, and given respecitvly interleukin-1 (IL-1) 100μ/ml, IL-1 100μ/ml +recombinant murine interleukin-10 (mnIL-10) 20ng/ml, mmIL-10 20ng/ml, and cultured for 48 hours. Scanning electron morphology and immunohistochemical study of nitric oxide synthease 2 and matric metalloproteinase 3 mRNA in situ hybridization were performed. Cell proliferation and morphology were observed under inverted microscope from the beginning of cell culture for three weeks. Results IL-1 stimulated granule production in the cytoplasma of chondrocytes, and the cells died in the second third weeks of culture. IL-10 antagonized IL-1, protected the cells from death and maintained chondrocyte proliferation. scanning electron morphology showed that IL-1 stimulated the formation of mumerous microvilli on the cell surface, while thin and less numerous microvilli were found in culturre with IL-10. Immunohistochemical study and in situ hybridization showed that IL-10 inhibited NOS2 and MMP3 expression. Conclusion IL-10 not only inhibits the synthesis of inflammatiory cytokines, but also directly protects chondrocytes by antagonizing IL-1.

关于软骨损伤后自我修复能务有限，不能形成正常透明软骨。人闪希望用细胞移植的方法修复软骨。本文介绍移植修复所涉及的细胞选择。载体系统选择，基质干细胞、载体材料的研究进展。

To study the effects of hydroxyurea and etoposide transduction of human marrkow mesenchymal and progenitor stem cells by adeno-associated virus (AAV). METHODS: Isolated human bone marrow mesenchymal stem and progenitor cells (hMSCs) were cultured in DMEM containing 10% FBS or 5% FBS and dexamethasone 1umol/L respectively. After being trated with hydroxyurea dn etoposide. hMSCs which indirectly reflected the relative trnasduction efficiency of different groups, and virus DNA was isolated by Hirt extracion for Southemhybridization. RESULTS: Transduction luciferase activity and transduction efficeiency in culture treadeted with hydroxyuread and etoposide were significantly higher than that in control cultures. Diveiding cells had about 20-fold hagher transduction efficeity compared with eontrol cells. Transduction efficeiency in stratinary cells was about 50 time higher than that in control cells. Southern analysis showed that hydroxyurea and etoposide enhanced second-trand DNA synthesis by rAAV. CONCUSION: Hydroxyurea and etoposide could increase trnadsuction efficiency of hMSCs by AAV vectors and stationalry cells were more sensitive to these than dividing cells.

AIM: To investigate the expression features of PDCD5 (programmed cell death 5 protein) in osteoarthritic and normal human cartilage, and speculate on its potential functions in the pathogenesis of osteoarthritis (OA). METHODS: Articular cartilage specimens were obtained from 30 patients with OA and 16 healthy patients at the time os arthroplasty. Expression of PDCD5 was detected by flow cytometry, immunofluorescence, RT-PCR and immunohistochemical analysis. RESULTS: Enhanced expression and nuclear accumulation of PDCD5 in OA chondrocytes were found. PDCD5-positive chondrocytes were mainly distributed in the superficial and deep zones of OA tissue sections, as opposed to, in the superficial and middle regions of normal healthy tissue sections.CONCLUSION: Since apoptotic chondrocyte death occurs more frequently in OA cartilage than in normal healthy cartilage and PDCD5 is an apoptosis-related protein, the different expression patterns of PDCD5 in OA cartilage from that in normal healthy cartilage indicate that PDCD5 is involved in the pathogenesis of OA.