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杨文才, 张晓敏, Francis David M
华北农学报,2009,24(4):183-187,-0001,():
-1年11月30日
为了明确我国主栽品种对番茄疮痂病抗性状况,本研究以我国番茄疮痂病主要小种T1和T3的菌株接种我国目前种植的39个番茄品种,并通过分子标记进行分析和辅助育种。结果表明,虽然有些品种具有了抗性材料的特征条带,但所有材料对T1和T3小种都不能产生过敏反应。采用与T1小种抗性位点脚紧密连锁的分子标记,成功地将抗病品种OH9834的抗性位点Rx3转移到9个不同的感病品种中,获得了抗病植株。从本研究可以看出,我国番茄品种缺乏对番茄疮痂病的抗性,同时也表明分子标记辅助选择可以用于番茄疮痂病的抗性育种中。
番茄, 疮痂病, 抗性鉴定, 分子标记辅助选择
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杨文才, Wencai Yang, Erik J. Sacks, Melanie L. Lewis Ivey, Sally A. Miller, and David M. Francis
Genetics and Resistance, 2005, 95 (5): 519-527,-0001,():
-1年11月30日
Yang, W., Sacks, E. J., Lewis Ivey, M. L., Miller, S. A., and Francis, D. M. 2005. Resistance in Lycopersicon esculentum intraspecific crosses to race T1 strains of Xanthomonas campestris pv. vesicatoria causing bacterial spot of tomato. Phytopathology 95: 519-527. We used molecular markers to identify quantitative trait loci (QTL) that confer resistance in the field to Xanthomonas campestris pv. vesica-toria race T1, a causal agent of bacterial spot of tomato. An F2 population derived from a cross between Hawaii 7998 (H 7998) and an elite breeding line, Ohio 88119, was used for the initial identification of an association between molecular markers and resistance as measured by bacterial populations in individual plants in the greenhouse. Polymorphism in this cross between a Lycopersicon esculentum donor of resistance and an elite L. esculentum parent was limited. The targeted use of a core set of 148 polymerase chain reaction-based markers that were identified as polymor-phic in L. esculentum × L. esculentum crosses resulted in the identifica-tion of 37 markers that were polymorphic for the cross of interest. Previous studies using an H 7998 × L. pennellii wide cross implicated three loci, Rx1, Rx2, and Rx3, in the hypersensitive response to T1 strains. Markers that we identified were linked to the Rx1 and Rx3 loci, but no markers were identified in the region of chromosome 1 where Rx2 is located. Single marker-trait analysis suggested that chromosome 5, near the Rx3 locus, contributed to reduced bacterial populations in lines carry-ing the locus from H 7998. The locus on chromosome 5 explained 25% of the phenotypic variation in bacterial populations developing in infected plants. An advanced backcross population and subsequent inbred back-cross lines developed using Ohio 88119 as a recurrent parent were used to confirm QTL associations detected in the F2 population. Markers on chromosome 5 explained 41% of the phenotypic variation for resistance in replicated field trials. In contrast, the Rx1 locus on chromosome 1 did not play a role in resistance to X. campestris pv. vesicatoria race T1 strains as measured by bacterial populations in the greenhouse or symp-toms in the field. A locus from H 7998 on chromosome 4 was associated with susceptibility to disease and explained 11% of the total phenotypic variation. Additional variation in resistance was explained by plant matur-ity (6%), with early maturing families expressing lower levels of resis-tance, and plant habit (6%), with indeterminate plants displaying more resistance. The markers linked to Rx3 will be useful in selection for resistance in elite × elite crosses.
advanced backcross breeding method.,
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杨文才
,-0001,():
-1年11月30日
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杨文才, Wencai Yang and David M. Francis
J. AMER. SOC. HORT. SCI. 130 (5): 716-721. 2005.,-0001,():
-1年11月30日
The lack of resistance to bacterial diseases increases both the fi nancial cost and environmental impact of tomato (Lycopersicon esculentum Mill.) production while reducing yield and quality. Because several bacterial diseases can be present in the same fi eld, developing varieties with resistance to multiple diseases is a desirable goal. Bacterial spot (caused by four Xanthomonas Dowson species) and bacterial speck (caused by Pseudomonas syringae pv. tomato Young, Dye and Wilkie) are two economically important diseases of tomato with a worldwide distribution. The resis-tance gene Pto confers a hypersensitive response (HR) to race 0 strains of the bacterial speck pathogen. The locus Rx3 explains up to 41% of the variation for resistance to bacterial spot race T1 in fi eld trials, and is associated with HR following infi ltration. Both Pto and Rx3 are linked in repulsion phase on chromosome 5. We made a cross between two elite breeding lines, Ohio 981205 carrying Pto and Ohio 9834 carrying Rx3, to develop an F2 population and subsequent inbred generations. Marker-assisted selection (MAS) was applied to the F2 progeny and to F2:3 families in order to select for coupling-phase resistance. Thirteen homozygous progeny from 419 F2 plants and 20 homozygous families from 3716 F3 plants were obtained. Resistance was confi rmed in all selected families based on HR in greenhouse screens using bacterial speck race 0 and bacterial spot race T1 isolates. Resistance to bacterial spot race T1 was confi rmed in the fi eld for 33 of the selected families. All selected families were also resistant to bacterial speck in the fi eld. MAS was an effi cient tool to select for desirable recombination events and pyramid resistance.
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【期刊论文】Identification of QTL associated with resistance to bacterial spot race T4 in tomato
杨文才
,-0001,():
-1年11月30日
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