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蔡俊鹏, Junpeng Cai, , Juan Li, Kim D. Thompson, Chuanxia Li and Hongcao Han
Journal of Basic Microbiology 2007, 47, 84-86,-0001,():
-1年11月30日
Mass mortality among the post-larvae of cultured abalone Haliotis diversicolor supertexta has occurred on the south coast of China since 2002. The diseased abalone are generally 10 to 30 days old, and typical signs of the disease include them turning white in colour and falling off the diatom films on which they were cultured. Among sixteen different motile bacteria isolated from the diseased post-larvae, four were identified as Vibrio parahaemolyticus on the basis of biochemical characteristics when compared with those of a V. parahaemolyticus type strain ATCC 17802T. Isolate 25, a representative isolate of V. parahaemolyticus recovered from diseased abalone, was virulent for the post-larvae with an LD50 value of 3.5 × 105 CFU (colony forming units)/ml. All moribund post-larvae artificially infected with the bacterium turned white and fell off the diatom films on which they were cultured as seen to occur during natural outbreaks of the disease, and it was possible to recover the bacterium from artificially infected post-larvae. The results of the study indicate that V. parahaemolyticus is a pathogenic bacterium to abalone post-larvae.
Mass mortality/, post-larvae/, abalone/, Vibrio parahaemolyticus/, pathogenic
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蔡俊鹏, 宋志萍, 蔡俊鹏*, 王志, 韩韫
微生物学报,2005,45(4):571~575,-0001,():
-1年11月30日
噬菌蛭弧菌具有裂解病原菌、净化水体的功效,从海洋环境中分离到4 株Bh04 系列蛭弧菌,对其生长条件进行了测定,同时研究了它们对61 株菌株的裂解能力。结果表明,在1%~3%的盐度范围内,蛭弧菌均可生长,最适盐度为3%!;在15~ 30!温度条件下蛭弧菌也可生长,但最适培养温度为20_25;只有在使用活的宿主菌的培养条件下,蛭弧菌才能生长。4株蛭弧菌分别可裂解21、24、4 0、43 株菌,各占总试验菌数(61)的344%、393%、656%和70 5%。4 株蛭弧菌一起,则可裂解55 菌株,占总试验菌株的902%。研究结果揭示了蛭弧菌在消除海洋环境中有害细菌方面的潜在应用价值。
蛭弧菌,, 病原菌,, 生长条件,, 裂解能力
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【期刊论文】一种消除九孔鲍苗细菌性病原的无公害绿色生物方法的研究
蔡俊鹏, 宋志萍, 王志, 韩韫
海洋科学,2006,30(1):44~48,-0001,():
-1年11月30日
从海洋环境中分离出4 株蛭弧菌(Bdellovibrio sp.),并对34 株九孔鲍 (Haliotis diversicolor) 苗细菌性病原进行了裂解试验。结果表明,Bh04-4、Bh04-41a、Bh04-A+和Bh04-1f 等4 株蛭弧菌分别可裂解11株、13 株、22 株和28 株病原菌裂解率为32.4%、38.2%、64.7%和87.5% 4;株共同作用则可裂解32 株病原菌,裂解率高达94.1%。研究结果展示了应用蛭弧菌控制九孔鲍苗细菌性病原的可行性。
蛭弧菌(, Bdellovibrio sp., ), , 裂解率, 九孔鲍 (, Haliotis diversicolor), 苗, 细菌性病原菌, 无公害绿色生物方法
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