To investigate the effects of human wild type p53 expression on the proliferation of cervical carcinoma cells, a plasmid, pM07 hp53, which contains a full-fength cDNA of the humao wild type p53 (wt p53) gene, was transfected into a cell line (TMCC 1) derived from an endocervical type, human papilloma virus positive adenocarcinoma of the uterine cervix. The exoge nous wt p53 expression induced growth suppression, morpho logical changes, and loss of anchorage independent growth of the tumor cells. As the wt p53 gene apparently plays a negative role in growth regulation of cervical carcinoma cells, this gene may possibly be of some use for treating subjects with a cervical carcinoma.

A consensus multiplex PCR rCM-PCR)technique was devel-oped to detect high-risk fHPV 16/18), low-risk(HPV 6/11), and over 40 other types of human papillomavirus(HPV), separately but simultaneously. by mixing three pairs of consensus primers in the same PCR mixture, for ene amplification. Simultaneous detection of three groups 0f HPV DNA provides valuable infor-matfon for clinical practice and this procedure is simple and convenient for routine laboratory examinations. We detected HPV DNA sequences in plasmid HPV DNA and DNA extracted from tissues of condyloma acuminata and cervical carcinoma and from exfoliated cells of the lower genital tract of healthy Chinese women living in the People’s Republic of China. We confirmed that this simple, convenient, and cost-beneficial CM-PCR technique is reliable for the detection of HPV DNA se-quences.