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贾金平, Le-hua Zhang a, ∗, Jin-ping Jia a, Di-wen Ying a, Nan-wen Zhu a, You-chun Zhu b
Research in Microbiology 156(2005)88-92,-0001,():
-1年11月30日
A bio-anode reactor and a bio-cathode reactor were developed to investigate the microenvironments around anodes and cathodes and their effects on denitrification. With an applied current of 40mA, the oxidation-reduction potentials (ORPs) in the bio-cathode and bio-anode reactors were 100-200 mV lower and 50mV higher, respectively, than that in the control reactor (a normal bio-reactor). The cathode reaction enhanced denitrification and the anode reaction inhibited denitrification. At 40 mA, the denitrification rate in the bio-cathode reactor was 55.1% higher than that in the control reactor. At 75mA, the denitrification rate in the bio-anode reactor was just 33.5% of that in control reactor. Electric current of less than 20mA had no effect on the most probable number (MPN) of denitrifiers, but at 75mA, the MPN of denitrifiers decreased by 90% in the bio-anode reactor. In the bio-cathode reactor, the MPN of denitrifiers increased more than 100% for the lower ORP environment produced by a cathode reaction at 75mA.
Bio-electro reactor, Denitrification, Denitrifier, Anode, Cathode, Electrode
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贾金平, Congying Gua, Jun Hea, Jinping Jia b, Nenghu Fangc, Robert Simmonsa, Shahab A. Shamsia, ∗
Journal of Chromatography A, 1217(2010)530-539,-0001,():
-1年11月30日
A surfactant-bound monolithic stationary phase based on the co-polymerization of 11-acrylaminoundecanoic acid (AAUA) is designed for capillary high performance liquid chromatography (HPLC). Using D-optimal design, the effect of the polymerization mixture (concentrations of monomer, crosslinker and porogens) on the chromatographic performance (resolution and analysis time) of the AAUA-EDMA monolithic column was evaluated. The polymerization mixture was optimized using three proteins as model test solutes. The D-optimal design indicates a strong dependence of chromatographic parameters on the concentration of porogens (1, 4-butanediol and water) in the polymerization mixture. Optimized solutions for fast separation and high resolution separation, respectively, were obtained using the proposed multivariate optimization. Differences less than 6.8% between the predicted and the experimental values in terms of resolution and retention time indeed confirmed that the proposed approach is practical. Using the optimized column, fast separation of proteins could be obtained in 2.5min, and a tryptic digest of myoglobin was successfully separated on the high resolution column. The physical properties (i.e., morphology, porosity and permeability) of the optimized monolithic column were thoroughly investigated. It appears that this surfactant-bound monolith may have a great potential as a new generation of capillary HPLC stationary phase.
11-Acrylaminoundecanoic acid (, AAUA), , D-optimal design, Monolithic column, Physical and chromatographic properties, Protein and protein digest separation
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【期刊论文】Study on a novel circulating cooling solid-phase microextraction method
贾金平, Liyan Liao, Ji Yang, Yalin Wang, Tonghua Sun, Jinping Jia ∗
Journal of Chromatography A, 1135(2006)1-5,-0001,():
-1年11月30日
A novel solid-phase microextraction (SPME) setup, circulating cooling solid-phase microextraction (CC-SPME), is developed for determining organochlorine pesticides (OCPs) in water. The linearity area of this method is 0.5-120g/l, its RSD value is less than 10% and detection limit is in the low ng/l when it is used to detect-hexachlorocyclohexane, which is better than traditional headspace SPME (HS-SPME) and direct immersion SPME (DI-SPME) methods. The influence of factors such as pH, ionic intensity, adsorption time, and adsorption temperature were also investigated, respectively.
SPME, Circulating cooling SPME, Organochlorine pesticides, Analytical method, ACF
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