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池振明, Lingmei Gao, Zhenming Chi, Jun Sheng, Lin Wang, Jing Li and Fang Gong
JmllD 248_ArtID 9231_Proof# 1-25/02/2007,-0001,():
-1年11月30日
Total 427 yeast strains from seawater, sediments, mud of salterns, guts of the marine fish, and marine algae were obtained. After inulinase activity of the yeast cultures was estimated, we found that four strains (OUC1, G7a, OUC2, and G7a1) of the marine yeasts grown in the medium with inulin could secrete a large amount of inulinase into the medium. The results of routine identification and molecular methods show that they belong to Pichia guilliermondii OUC1, Cryptococcus aureus G7a, Yarrowia lipolytica OUC2, and Debaryomyces hansenii G7a1, respectively. The optimal pHs of inulinase activity produced by them were 6.0, 5.0, 5.0, and 5.0, respectively, while the optimal temperatures of inulinase activity produced by them were 60-, 50-, 60-, and 50-C, respectively. A large amount of monosaccharides and a trace amount of oligosaccharides were detected after the hydrolysis by the crude inulinase produced by P. guilliermondii OUC1, indicating that the crude inulinase had a high exoinulinase activity while a large amount of monosaccharides and oligosaccharides were detected after inulin hydrolysis by the crude inulinase produced both by C. aureus G7a and D. hansenii G7a1. However, no monosaccharides and disaccharides were detected after inulin hydrolysis by the crude inulinase produced by Y. lipolytica OUC2, suggesting that the crude inulinase had no exoinulinase activity.
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池振明, Jun Sheng, Zhenming Chi, Jing Li, Lingmei Gao, Fang Gong
J. Sheng et al. Process Biochemistry xxx (2007) xxx-xxx,-0001,():
-1年11月30日
The marine yeast strain G7a isolated from sediment of China South Sea was found to secrete a large amount of inulinase into the medium. This marine yeast strain was identified to be a strain of Cryptococcus aureus according to the results of routine yeast identification and molecular methods. The crude inulinase produced by this marine yeast showed the highest activity at pH 5.0 and 50 8C. The optimal medium for inulinase production was artificial seawater containing inulin 4.0% (w/v), K2HPO4 0.3% (w/v), yeast extract 0.5% (w/v), KCl 0.5% (w/v), CaCl2 0.12% (w/v), NaCl 4.0% (w/v) and MgCl2
Inulinase, Marine yeasts, Cryptococcus aureus, Inulin hydrolysis
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池振明, Fang
J Ind Microbiol Biotechnol (2007) 34: 179-185,-0001,():
-1年11月30日
Marine yeast strain 1, isolated from the surface of a marine alga, was found to secrete a large amount of inulinase into the medium. This marine yeast was identiWed as a strain of Pichia guilliermondii according to the results of routine yeast identiWcation and molecular methods. The crude inulinase produced by this marine yeast worked optimally at pH 6.0 and 60
Inulinase, Marine yeasts, Marine environment, Pichia guilliermondii, 18S rDNA, ITS
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池振明, Zhen-Ming CHI, Jun-Feng LI, Xiang-Hong WANG, and Shu-Min YAO
Acta Biochimica et Biophysica Sinica 2004, 36 (7): 443-449,-0001,():
-1年11月30日
Glucose repression occurs in many yeast species and some filamentous fungi, and it represses the expression and secretion of many intracellular and extracellular proteins. In recent years, it has been found that many biochemical reactions in yeast cells are mediated by phosphatidylinositol (PI)-type signaling pathway. However, little is known about the relationships between PI-type signaling and glucose repression, gene expression and invertase secretion in yeasts. Many evidences in our previous studies showed that glucose repression, invertase secretion, gene expression and cell growth were mediated by inositol and PI in Saccharomyces and Schizosaccharomyces. The elucidation of the new regulatory mechanisms of protein secretion, gene expression and glucose repression would be an entirely new aspect of inositol and PI-type signaling regulation in yeasts.
glucose repression, PI-type signaling pathway, invertase secretion, gene expression
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池振明, Haifeng
H. Li et al. Process Biochemistry 42 (2007) 462-465,-0001,():
-1年11月30日
Under the optimal conditions, 10 U/ml of glucoamylase was produced by the marine yeast Aureobasidium pullulans N13d. It was noticed that the crude glucoamylase actively hydrolyzed potato starch granules, but poorly digested raw corn starch and sweet potato starch, resulting in conversion of 68.5, 19 and 22% of them into glucose within 6 h of incubation in the presence of 40 g/l of potato starch granules and 20 U/ml of the crude enzyme. When potato starch granules concentration was increased from 10 to 80 g/l, hydrolysis extent was decreased from 85.6 to 60%, while potato starch granules concentration was increased from 80 to 360 g/l, hydrolysis extent was decreased from 60 to 56%. Ratio of hydrolysis extent of potato starch granules to hydrolysis extent of gelatinized potato starch was 86.0% and the hydrolysis extent of potato starch granules by action of the crude glucoamylase (1.0 U/ml) was 18.5% within 30 min at 60 8C. Only glucose was detected during the hydrolysis, indicating that the crude enzyme could hydrolyze both a-1,4 and a-1,6 linkages of starch molecule in the potato starch.
Aureobasidium pullulans, Marine yeast, Glucoamylase, Potato starch granules
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