目的 研究不阻断肝门的肝切除术在大肝癌切除手术中的价值。方法 回顾性分析30例不阻断肝门的大肝癌切除术，并与同期98例采用肝门阻断的大肝癌切除术做对比。采用单因素和多因素分析的方法，研究与大肝癌术后并发症有关的因素。结果 不阻断肝门组术后并发症率低于阻断肝门组（1010% vs 3217%，P=0102）。单因素分析显示年龄、肝门阻断、术中出血量、输血量以及手术时间等与并发症发生有关，进一步通过多元逐步回归模型分析发现，年龄、肝门阻断、输血量以及手术时间是决定术后并发症发生的4个独立的预测指标。结论 大肝癌切除手术中有选择性地采用不阻断肝门的肝切除技术是安全可行的。

目的 研究肝细胞癌（HCC）中血管内皮生长因子（VEGF）、缺氧诱生因子1 alpha（HIF-1α）和表皮生长因子（EGF）的表达情况及其临床意义。方法 采用免疫组织化学SABC法检测36例HCC组织及其癌旁肝组织和6例正常肝组织中VEGF、HIF-1α和EGF的表达情况，研究这3个因子与HCC临床病理学资料、新生血管生成以及预后的关系。结果 36例HCC中VEGF、HIF-1α和EGF表达的阳性率分别为89%（32/36），67%（24/36）和75%（27/36），均高于相应的癌旁肝组织和正常肝组织（P<0.05）。光学显微镜下有静脉浸润的HCC组织中VEGF和HIF-1α的表达率分别为96%（23/24）和88%（21/24），高于光学显微镜下无静脉浸润者（75%和25%），差异有显著意义（P<0.05）。VEGF阴性组术后1、2年生存率均为100%，弱阳性组分别为87%和22%，强阳性组分别为54%和0，三组存活率的差异具有显著意义（P<0.05）。EGF 阴性组术后1、2年存活率分别为100%和60%，弱阳性组均为70%，强阳性组分别为27%和0、三组之间存活率的差异亦具有显著意义（P<0.05）。结论 HCC组织中VEGF、HIF-1α和EGF呈过量表达。HCC组织中VEGF、EGF和HIF-1α的表达与HCC中新生血管生成以及预后不良有密切关系。

AIM: To investigate the role of tri-iodothyronine supplement in protecting gut barrier in septic rats. METHODS: Twenty-two rats were randomized into three groups: sham group (n=6), sepsis group (n=8), and sepsis plus tri-iodothyronine (T3) group (n=8). Septic rat model was established through cecal ligation and puncture (CLP). After 5 h, sham and sepsis groups received saline, and the remaining group received T3 intraperitoneally. Twenty-one hrs After CLP, intestinal permeability and serum free T3 and T4 were measured with fluorescence spectrophotometer and by radioimmunoassay, respectively. Intestinal ultrastructure and histologic morphology were observed under transmission electron microscopy (TEM) and light microscopy, respectively. RESULTS: After 21 h, septic symptoms and signs in sepsis plus T3 group were milder than those in sepsis group. Serum FT3 or FT4 concentration in sepsis group was lower than that in sham group (1.59

目的 研究探讨RhoC基因mRNA和蛋白的过量表达与肝细胞癌（HCC）的发生发展和侵袭转移的关系。方法 采用逆转录聚合酶链反应（RT2PCR）和Western印迹法，检测25例HCC及其癌旁肝组织、4例门静脉主干癌栓或肝外转移灶RhoCmRNA和蛋白的表达；同时采用PCR产物单链构象多态性（PCR2SSCP）银染法检测RhoC基因的突变情况。结果 HCC组织和癌旁肝组织中均可检测到RhoC基因mRNA和蛋白的表达， RhoCmRNA在HCC组织中的表达较癌旁肝组织高，其吸光度（A）比值分别为1.8±1.1和1.0±0.7，差异有显著性（P<0.01）；RhoC蛋白在HCC组织中的表达同样高于癌旁肝组织，其A比值分别为33992±10384和17342±9998， 差异有显著性（P<0.01）。4例门静脉主干癌栓或肝外转移灶中的RhoC基因mRNA和蛋白的表达量分别为3.3±0.5和63865±9116，较HCC肝内病灶高，差异有显著性（P<0.01）；RhoCmRNA和蛋白在分化较差、结节数较多、有静脉浸润或伴有肝外转移灶的HCC中过量表达（P<0.05）。全部HCCCR2SSCP银染法分析均未发现异常泳动条带。结论 RhoC基因的过量表达与HCC的发生发展和侵袭转移密切相关，且可能是通过过量表达而非突变发挥作用。

AIM: To investigate the expression of metallothioneins (MTs), which were recently thought to have close relationship with tumors, in human hepatocellular carcinoma. METHODS: Histological specimens of 35 cases of primary human hepatocellular carcinoma with para-neoplastic liver tissue and 5 cases of normal liver were stained for MTs with monoclonal mouse anti-MTs serum (E9) by the immunohistochemical ABC technique. RESULTS: MTs were stained in the 35 cases of HCC, including 6 cases negative (17.1%), 23 weakly positive (65.7%), and 6 strongly positive(17.1%). But MTs were stained strongly positive in all the five cases of normal liver and 35 cases of para-neoplastic liver tissue. The differences of MTs expression between HCC and normal liver tissue or para-neoplastic liver tissue were highly significant (P<0.01). The rate of MTs expression in HCC grade I was 100 percent, higher than that in grade II(81%) and grade III and IV (78%). But the differences were not significant (P>0.05). No obvious correlations between MTs expression in HCC and tumor size, clinical stage or serum alpha fetoprotein concentration were found (P>0.05). CONCLUSION: Decrease of MTs expression in HCC may play a role in carcinogenesis of HCC. MTs are stained heterogenously in HCC. We can choose the anticancer agents according to the MTs concentration in HCC, which may improve the results of chemotherapy for HCC.