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周丛照, Jianping Sun,
Biochemistry 2005, 44, 8801-8809,-0001,():
-1年11月30日
Kti11p is a small, highly conserved CSL zinc finger-containing protein found in many eukaryotes. It was first identified as one of the factors required for maintaining the sensitivity of Saccharomyces cereVisiae to KluyVeromyces lactis zymocin. Then, it was found to be identical to Dph3, a protein required for diphthamide biosynthesis on eEF-2, the target of diphtheria toxin and Pseudomonas exotoxin A, in both yeast and higher eukaryotes. Furthermore, Kti11p/Dph3 was found to physically interact with core-Elongator, ribosomal proteins, eEF-2, two other proteins required for diphthamide modification on eEF-2, and DelGEF. Here, we determined the solution structure of Kti11p using NMR, providing the first structure of the CSL-class zinc-binding protein family. We present the first experimental evidence that Kti11p can bind a single Zn2+ ion by its four conserved cysteine residues. The major structure of Kti11p comprises a ‚ sandwich as well as an R helix. Moreover, a structure-based similarity search suggests that it represents a novel structure and may define a new family of the zinc ribbon fold group. Therefore, our work provides a molecular basis for further understanding the multiple functions of Kti11p/Dph3 in different biological processes.
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周丛照, Marc Graille‡§, Cong-Zhao Zhou§¶, Veronique Receveur-Brechot**, Bruno Collinet¶, Nathalie Declerck‡‡§§, and Herman van Tilbeurgh‡¶¶
THE JOURNAL OF BIOLOGICAL CHEMISTRY 280(15)14780-14789, 2005,-0001,():
-1年11月30日
The transcriptional antiterminator protein Lic Tregulates the expression of Bacillus subtilis operons involved in-glucoside metabolism. It consists of an Nterminal RNA-binding domain (co-antiterminator (CAT)) and two phosphorylatable phosphotransferase system regulation domains (PRD1 and PRD2). In the activated state, each PRD forms a dimericunit with the phosphorylation sites totally buried at the dimer interface. Here we present the 1.95
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周丛照, CONG-ZHAO ZHOU, , PHILIPPE MEYER, SOPHIE QUEVILLON-CHERUEL, IN
Protein Science (2005), 14: 209-215. Published by Cold Spring Harbor Laboratory Press. Copyright,-0001,():
-1年11月30日
We determined the three-dimensional crystal structure of the protein YML079wp, encoded by a hypothetical open reading frame from Saccharomyces cerevisiae to a resolution of 1.75
jelly-roll motif, cupin superfamily, structural genomics, YML079wp, S., cerevisiae
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