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【期刊论文】The Preparation and Bioactivity Research of Agaro-Oligosaccharides
严小军, Hai-Min Chen, Li Zheng and Xiao-Jun Yan*
H.-M. CHEN et al.: Agaro-Bioactivity Research of Oligosaccharides, Food Technol. Biotechnol. 43(1)29-36 (2005),-0001,():
-1年11月30日
Agaro-oligosaccharides were hydrolytically obtained from agar using hydrochloric acid, citric acid, and cationic exchange resin (solid acid). The FT-IR and NMR data showed that the hydrolysate has the structure of agaro-oligomers. Orthogonal matrix method was applied to optimize the preparation conditions based on α-naphthylamine end-labeled HPLC analysis method. The optimal way for oligosaccharides with different degree of polymerization (DP) was achieved by using solid acid degradation, which could give high yield and avoid solution neutralization process. Agaro-oligosaccharides with high purity were consequently obtained by activated carbon column isolation. Furthermore, the antioxidant and α-glucosidase inhibitory activity of three fractions were also investigated. The result indicated that 8% ethanol-eluted fraction showed highest activity againstα-glucosidase with IC50 of 8.84mg/mL, while 25% ethanol-eluted fraction possessed excellent antioxidant ability.
agaro-oligosaccharides,, orthogonal matrix,, HPLC,, acid,, degradation,, antioxidant,, α-glucosidase
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【期刊论文】Hymeniacidon perleve Associated Bioactive Bacterium Pseudomonas sp. NJ6-3-1*
严小军, Li Zheng, Xiaojun Yan, Jilin Xu, Haimin Chen, and Wei Lin
Applied Biochemistry and Microbiology, Vol. 41, No.1, 2005, pp. 29-33.,-0001,():
-1年11月30日
Among marine bacteria isolated from the cytotoxic sponge Hymeniacidon perleve, one strain NJ6-3-1 classified as Pseudomonas sp. showed both cytotoxic and antimicrobial activities. Fatty acid analysis indicated that the bacterial strain consists mainly of C16:1, C16:0, C18:1, C18:0, C15:0, C14:0. One unusual 9, 10-cyclopropane-C17:0 fatty acid and C26:0 also constitute major components, as well as the existence of squalene, the precursor of triterpenoids. The major metabolites in the culture broth were identified as alkaloids, including diketopiperazines and indole compounds, namely 3,6-diisopropylpiperazine-2,5-dione, 3-benzyl-3-isopropylpiperazine-2,5-dione, 3,6-bis-(2-methylpropyl)-piperazine-2,5-dione, indole-3-carboxaldehyde, indole-3-carboxylic acid methyl ester, indole-3-ethanol, and quinazoline-2,4-dione.
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