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万有, Huiyin Tu, Lunbin Deng, Qian Sun, Lei Yao, Ji-Sheng Han, and You Wan*
Journal of Neuroscience Research 76: 713-722 (2004),-0001,():
-1年11月30日
The large, medium-sized, and small neurons of the dorsal root ganglion (DRG) have different functions in the processing of various senses. Hyperpolarization-activated, cyclic nucleotide-gated channels (HCN) contribute greatly to neuronal excitability. In the present study, which used whole-cell patch clamp techniques and immunohistochemical staining methods, the electrophysiological properties of DRG neurons were systematically compared, and the roles of HCN-1, -2, and -4 were examined. The main results were as follows. 1) The large neurons had significantly higher V0.5 values (membranepotential at which the HCN channels were half-activated) and shorter time constants (HCN) than small or mediumsized DRG neurons. However, large DRG neurons had higher Ih density (HCN neuron current). 2) HCN-1 was found predominantly, but not exclusively, in large and medium-sized DRG neurons; HCN-2 was found in all DRG neurons; and HCN-4 was poorly visualized in all DRG neurons. HCN-1 and HCN-2 were colocalized in large and medium-sized neurons with immunostaining of adjacent sections. In the dorsal horn of the spinal cord, HCN-1, HCN-2, and HCN-4 were all expressed in laminae I-IV, although HCN-1 was not detectable in lamina II. 3) Blockade of Ih current in DRG neurons caused a signi ficant decrease in V0.5, resting membrane potential, and repetitive firing number of action potential and a significant increase in time of rising phase of action potential. These results suggest that the different HCN channels in the three types of DRG neurons might contribute to their differential electrophysiological properties.
hyperpolarization-activated, cyclic nucleotidegated channel, Ih, dorsal root ganglion, ZD7288
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万有, Qian Sun, Huiyin Tu, Guo-Gang Xing, Ji-Sheng Han, You Wan*
Experimental Neurology 191(2005)128-136,-0001,():
-1年11月30日
It is widely accepted that ectopic discharges originated from injured sites and dorsal root ganglion (DRG) neurons after peripheral nerve injury contribute to neuropathic pain. However, it has been recently shown that ectopic discharges were not always necessary for neuropathic pain. In the present study, we aim to further examine the role of ectopic discharges in neuropathic pain in a spinal nerve ligation (SNL) model. With teased fiber recordings in vivo, the characteristics of ectopic discharges were observed over 14 days after SNL, and the correlation between ectopic discharges and tactile allodynia was analyzed. It was observed that ectopic discharges have three firing patterns (tonic, bursting, and irregular) after SNL, and proportions of these three patterns changed dynamically over time. The tonic and bursting types were dominant in the first 24h following SNL, while the irregular type became the only pattern in the late stage (day14). The average frequencies of ectopic discharges and the percentage of active filaments also changed over time, reaching the peak 24h after SNL and then declined gradually. Ectopic discharges were highly correlated with tactile allodynia in the first 24h following SNL, but surprisingly, not in the late stage of days 1 to 14. These findings suggest that ectopic discharges may be crucial in the triggering of neuropathic pain in the early stage, but their importance become more limited over time.
Ectopic discharge, Dorsal root ganglion, Neuropathic pain, Spinal nerve ligation, Teased fiber recording, Tactile allodynia
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万有, Hao Luo, Jin Cheng, Ji-Sheng Han and You WanCA
Vol. 15 No.4 22 March 2004,-0001,():
-1年11月30日
The present study aimed to systematically observe the change of vanilloidreceptor1 (VR1) during in
Complete Freund', s adjuvant, Dorsal root ganglion, In
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万有, Cheng Huang, Yun Wang, Ji-Sheng Han, You Wan*
Brain Research 945(2002)20-25,-0001,():
-1年11月30日
The present study was conducted to evaluate the characteristics of electroacupuncture (EA)-induced analgesia in mice. Three inbred strains of mice (DBA/2, C57BL/6J, BALB/c) and three outbred strains (ICR, LACA, NIH) were used in the experiment. Two pairs of metallic needles were inserted into acupoints ST 36 and SP 6 connected to an electric pulse generator. EA parameters were set as constant current output with alteration of a positive and negative square wave, 0.6ms in pulse width for 2Hz and 0.3ms for 100Hz. Tail-flick latencies evoked by radiant heat were measured before, during and after EA stimulation. We found that (1) DBA/2 mice showed a significantly more potent analgesic effect than the other five strains in response to both 100 and 2Hz EA. In this case, the intensities were 1.0-2.0-2.0mA, 10 min for each intensity totally 30min. (2) EA analgesia increased as the intensity of stimulation increased from 0.5 to 21 2.0mA, but it remained at this plateau when the intensity further increased from 2.0 to 3.0mA. (3) 10.0mg?kg naloxone was needed to 21 block the analgesic effect induced by 2Hz EA of 2.0mA, but to block that by 100Hz, 25.0mg?kg was necessary. (4) A positive 21 correlation was observed between analgesia induced by morphine at the dose of 5.0mg?kg and by 100Hz EA in two tested strains DBA/2 and C57BL/6J. In conclusion, EA induces reliable, strain-dependent analgesia in mice. The naloxone-reversibility of EA, a measure of whether it is opioid or non-opioid mediated, is dependent upon intensity and frequency.
Analgesia, Electroacupuncture, Strain, Morphine, Naloxone
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【期刊论文】Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury
万有, Xiao-Qing Tang, Yun Wang, Ji-Sheng Han and You WanCA
Vol. 12 No.14 8 October 2001,-0001,():
-1年11月30日
The protective effects of adenovirus-mediated glia cell linederived neurotrophic factor (GDNF) gene transaction was investigated on cultured motoneurons. First, the dose
Adenovirus, Excitatory amino acid, Gene therapy, Glia cell line-derived neurotrophic factor, Glutamate, Motoneuron, Spinal cord
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