介绍了一种从木本植物组织中获得高质量RNA 的快速、简单和高效的核酸提取方法。该方法是基于核酸的二氧化硅捕获，避免了使用苯酚、氯仿等有机溶剂。利用该方法从樱桃组织中提取的总RNA 用RT 2PCR技术检测PDV，PNRSV 均获得成功。从感病植株的一年生枝的叶片、韧皮部及芽组织中扩增出了预期的目的片段，即172和449bp，而健康组织中无此扩增带。该法提取的总RNA用于RT 2PCR技术检测，其敏感性至少与商业出售的Qiagen RNeasy提取试剂盒相当，但简单经济。

Approximately 80 days after full bloom, well-exposed fruit on the south part of the canopy of mature Liberty/M9 apple (Malus domestica Borkh.) trees were randomly assigned to one of the following two treatments. Some fruits were turned about 180◦ to expose the original shaded side to full sun, whereas the rest served as untreated controls. On days 0, 1, 2, 4, 7 and 10 after treatment, fruit peel samples were taken from the original shaded side of the treated fruit and both the sun-exposed side and the shaded side of the control fruit at midday, to determine photosynthetic pigments, enzymatic and non-enzymatic antioxidants. Maximum photosystem II (PSII) efficiency, measured as Fv/Fm (maximum fluorescence) of chlorophyll fluorescence at pre-dawn, was higher in the shaded side than the sun-exposed side of the control fruit. Fv/Fm of the original shaded side decreased sharply after 1 day exposure to full sun, and then gradually recovered to a similar value of the sun-exposed side of the control fruit by day 10. The shaded side of the control fruit had much lower xanthophyll cycle pool size, conversion and antioxidant enzymes and also soluble antioxidants of the ascorbate–glutathione cycle than the sun-exposed side. Xanthophyll cycle pool size of the original shaded side increased in response to full sun exposure, reaching a similar value of the sun-exposed side by day 10. Almost all the xanthophyll cycle pool was converted to zeaxanthin and antheraxanthin starting from the first day of exposure to full sun. In contrast, chlorophyll content decreased whereas lutein or neoxanthin content remained unchanged. In response to full sun exposure, ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase and total pool size and reduction state of both ascorbate and glutathione, all increased to the corresponding values found in the sun-exposed side of the control fruit over a 10-day period. It is concluded that both xanthophyll cycle and the ascorbate-glutathione cycle in the original shaded side are up-regulated in response to full sun exposure to minimize photo-oxidative damage and contributes to its acclimation to full sun.

在45℃下对猕猴桃一年生盆栽苗进行2，4，6，8，10h的高温胁迫，测定高温胁迫下叶绿素含量、相对膜透性、丙二醛（MDA）含量、抗氧化酶活性和抗氧化剂含量及热激蛋白等生理生化指标。结果表明，高温胁迫下叶绿素含量下降，相对膜透性增大，MDA 含量增加； 超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸还原酶（APX）活性均在胁迫初期呈上升趋势，随处理时间延长，活性下降；随胁迫时间延长，还原型谷胱甘肽（GSH） 含量先升后降，抗坏血酸（ASA）含量总体呈下降趋势，谷胱甘肽还原酶（GR）和脯氨酸含量一直呈上升趋势；热激蛋白（HSP）在高温下被诱导。

梨树（Pyrus bretschneideri Regd.）自然休眠和休眠解除时，花芽的活性氧代谢发生变化。O2-·产生速率和H2O2的含量在休眠期间上升，在休眠后期下降。抗氧化系统中SOD活性在自然休眠期呈下降趋势，自然休眠结束活性上升。POD和CAT活性在自然休眠期上升。抗氧化物质ASA和GSH的含量随休眠进行而下降，休眠解除过程中重新升高。APX和GR的活性在休眠期间活性下降，休眠结束活性迅速上升。这些结果表明：花芽的休眠与活性氧的代谢有很大关系。

用梨（Pyrus）4个栽培品种的叶片诱导不定芽。暗培养20d，取试管苗顶部幼嫩叶片，远轴面向下接触培养基，叶片再生效率高。八月红品种的叶片再生效率高于其它3个品种。在NN69+TDZ 1.0mg/L+IBA 0.5mg/L 培养基上，八月红梨叶片再生频率为100%，平均再生芽数为5.78。八月红梨和水晶梨叶片再生苗在附加IBA的1/2MS培养基上生根。