已为您找到该学者16条结果 成果回收站
夏光敏, Ai Xia CHENG, Guang Min XIA*, Da Ying ZHI, Hui Min CHEN
,-0001,():
-1年11月30日
We report the production and characterization of somatic hybrids between Triticum aestivum L. and Agropyron elongatum (Host) Nevishi (the synonym is Thinopyrum ponticum). Asymmetric protoplast fusion was performed between Agropyron elongatum protoplasts irradiated with a low UV dose and protoplasts of wheat taken from nonregenerable suspension cultures. More than 40 green plantlets were obtained from 15 regenerated clones and one of them produced seeds. The phenotypes of the hybrid plants and seeds were intermediate between wheat and Agropyron elongatum. All of the regenerated calli and plants were verified as intergeneric hybrids on the basis of morphological observation and analysis of isozyme, cytological, 5SrDNA spacer sequences and random amplified polymorphic DNA (RAPD). RFLP analysis of the mitochondrial genome revealed evidence of random segregation and recombination of mtDNA.
Triticum aestivum,, Agropyron elongatum,, somatic hybridization,, mitochondrial genome.,
-
66浏览
-
0点赞
-
0收藏
-
0分享
-
166下载
-
0
-
引用
【期刊论文】Agropyron elongatum chromatin localization on the wheat chromosomes in an introgression line
夏光敏, Jing Wang
Planta (2005) 221: 277-286,-0001,():
-1年11月30日
The introgressed small-chromosome segment of Agropyron elongatum (Host.) Neviski (Thinopyrum ponticum Podp.) in F5 line II-1-3 of somatic hybrid between common wheat (Triticum aestivum L.) and A. elongatum was localized by sequential fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and karyotype data. Karyotype analysis offered basic data of arm ratios and relative lengths of 21 pairs of chromosomes in parent wheat Jinan177 and hybrid II-1–3. Using special high repetitive sequences pSc119.2 and pAs1 for FISH, the entire B- and D-genome chromosomes were detected. The FISH pattern of hybrid Ⅱ-1-3 was the same as that of parent wheat. GISH using whole genomic DNA from A. elongatum as probe determined the alien chromatin. Sequential GISH and FISH, in combination with some of the karyotype data, localized the small chromosome segments of A. elongatum on the specific sites of wheat chromosomes 2AL, 1BL, 5BS, 1DL, 2DL and 6DS. FISH with probe OPF-031296 from randomly amplified polymorphic DNA (RAPD) detected E-genome chromatin of A. elongatum, which existed in all of the small chromosome segments introgressed. Microsatellite primers characteristic for the chromosome arms above were used to check the localization and reveal the genetic identity. These methods are complementary and provide comprehensive information about the genomic constitution of the hybrid. The relationship between hybrid traits and alien chromatin was discussed.
Agropyron elongatum
-
57浏览
-
0点赞
-
0收藏
-
0分享
-
104下载
-
0
-
引用
夏光敏, Chunhui Xu, Guangmin Xia*, Daying Zhi, Fengning Xiang, Huimin Chen
Plant Science 165 (2003) 1001-1008,-0001,():
-1年11月30日
Protoplasts were isolated from cultured cells of wheat and maize and fused using PEG. Calli and green plants were regenerated following irradiation of the maize, and some tested positive for hybridity using morphological, isozymic and various DNA-based marker systems. Genomic in situ hybridization (GISH) of selected maize-carrying regenerants showed that some maize chromatin was dispersed throughout the wheat nuclear genome. Cytogenomic analysis, using RFLP and SSR, demonstrated the presence of both wheat and maize loci in the mitochondrial genome, so that, in the hybrid individuals, either distinct mitochondria from each parent coexist and/or recombinant mitochondria have been generated. With respect to the chloroplast genome, there was no evidence of the presence of any introgression from maize.
Wheat, Maize, Asymmetric somatic hybridization, Nuclear genome, Cytogenome, Integration
-
55浏览
-
0点赞
-
0收藏
-
0分享
-
96下载
-
0
-
引用
【期刊论文】LMW-GS genes in Agropyron elongatum and their potential value in wheat breeding
夏光敏, Zhen Luo
Theor Appl Genet (2005) 111: 272-280,-0001,():
-1年11月30日
To study the usefulness of low-molecularweight glutenin subunits (LMW-GS) of Agropyron elongatum (Host) Nevski to wheat (Triticum aestivum L.) quality improvement, we characterized LMW-GS genes of A. elongatum. Nine LMW-GS genes of A. elongatum, which were named AeL1 to AeL9, were cloned by genomic PCR. After sequencing, we obtained complete open reading frames from AeL2 to AeL8 and partial genes of AeL1 and AeL9. All nine sequences are homoeologous to those of wheat and related grasses. Comparison of the deduced amino acid sequences with those of published LMW-GS suggests that the basic structures of all the subunits are very similar. However, except for AeL4 and AeL5, which contain the identical N-terminal sequence with LMW-m, other LMW-GS sequences separated from A. elongatum cannot be classified according to previous criteria for the three types: LMW-m (methionine), LMW-s (serine), and LMW-i (isoleucine), and then 12 groups. In addition, there are some characters in the LMW-GS sequences of A. elongatum: AeL2, AeL3, and AeL6 involve a Cys residue in the signal peptide respectively, which is absent in most of LMW-GS; AeL3, AeL6, AeL8, and AeL9 start their first Cys residues in the N-terminal repetitive domains, respectively; both AeL2 and AeL5 have nine Cys residues, with an extra Cys residue in the N-terminal repetitive domain and the repetitive and glutamine-rich domain; AeL2, AeL3, AeL6, and AeL9 comprise long repetitive domains. Phylogenetic analysis indicates that there is a relatively weak sequence identity between the LMW-GS genes from A. elongatum cloned in this study and those reported from other plants. Three LMW-GS sequences, AeL2, AeL3, and AeL6, are clustered to Glu-A3 from wheat than to those from other plants. The possible use of these genes in relation to the high quality of hybrid wheat is discussed.
Agropyron elongatum
-
54浏览
-
0点赞
-
0收藏
-
0分享
-
83下载
-
0
-
引用
夏光敏, Chen Suiyun, Xia Guangmin*, Quan Taiyong, Xiang Fengnin, Jin Yan, Chen Huimin
C. Suiyun et al./Plant Science xxx (2004) xxx-xxx,-0001,():
-1年11月30日
Introgression lines derived from somatic hybrids between Thinopyrum ponticum Podp. (Agropyron elongatum (Host) Nevishi) and Triticum aestivum L. cv. Jinan 177 were screened for salt-tolerance in hydroponic experiments. Their growth rates, salt-tolerance index and the content of free proline, Na+, K+ and Na+/K+ in the leaf were compared with those of wheat cv. Jinan 177. The lines were tested under natural saline conditions in two locations. One line expressed higher salt-tolerance than its parental wheat and a check salt-tolerant cultivar. Karyotype analysis showed that the two lines possessed 42 chromosomes of wheat introgressed with small chromosome segments from Th. ponticum. We suggest that these two lines show stable inheritance of salt tolerance, and that this salt-tolerance has been transferred from Th. ponticum into wheat.
Somatic hybrid lines, Triticum aestivum L., , Thinopyrum ponticum (, Agropyron elongatum), , Salt-tolerance, GISH
-
54浏览
-
0点赞
-
0收藏
-
0分享
-
68下载
-
0
-
引用