目的进行颞下颌关节滑膜间充质干细胞（SMSCs）的体外分离与培养。方法用有限稀释法筛选出SMSCs，并在25ml培养瓶中扩增培养。噻唑蓝法检测SMSCs的细胞生长周期，细胞免疫化学检测波形蛋白和角蛋白的表达，流式细胞技术对SMSCs进行CD8，CD34，CD44，血管细胞间黏附分子-1（VCAM-1）和细胞间黏附分子-1（ICAM-1）的检测。结果体外培养的SMSCs，细胞突起明显，细胞外形均匀一致，免疫染色显示波菜蛋白阳性与角蛋白阴性。流式细胞仪检测发现其有CD44、VCAM-1和ICAM-1的表达。结论从成人的颞下颌关节滑膜中可以分离培养出SMSCs。

目的：观察颞下颌关节盘前移位动物模型和临床标本的超微结构改变，了解关节盘前移位的病理变化过程。方法：手术使兔颞下颌关节盘前移位，在术后2，4，8，12周应用透射电镜观察实验动物关节盘的病理性改变同时在临床上取颞下颌关节盘前移位的标本进行相同研究。结果：在关节盘前移位早期的标本中，关节盘胶原纤维排列紊乱，纤维断裂，软骨细胞粗面内质网扩张、线粒体肿胀。后期出现软骨细胞的坏死，细胞胞浆内有扩张的粗面内质网、大量微丝、空泡，关节盘胶原纤维排列紊乱、并出现弹力纤维。结论：颞下颌关节盘前移位早期出现关节盘的退行性改变，后期关节组织病变与修复同时存在。

The purpose of this study was to investigate the waveform and electrosonographic characteristics of sounds emanating from internal derangement of the temporomandibular joint (TMJ). TMJ sounds were ecorded from 10 joints of normal people (NP), 10 joints from patients with anterior disc isplacement with reduction (DDR) and 20 joints from patients with anterior disc displacement without reduction (DDNR). The sounds were analyzed through fast Fourier transfer methods to observe their waveforms and electrosonographic characteristics. The observations were then used in differentially diagnosing internal derangement. Wave pattern and electrosonography (ESG) differed among the NP, DDR and DDNR groups. There was very little difference in frequency between the sounds from DDR and DDNR, but the amplitude of the DDR sounds was higher than those of DDNR and NP. The sensitivity and specificity of ESG diagnosis for DDR were 77.2% and 93.3%, respectively, while for DDNR they were 81.6% and 64.7%, respectively.

To measure the activity of oxygen free radicals and the level of antioxidant enzyme superoxide dismutase (SOD) in the synovial fluid (SF) of the temporomandibular joints (TMJs) of patients with temporomandibular disorders (TMD). Methods: Thirty-two patients were divided into 3 subgroups: anterior disc displacement with reduction, anterior disc displacement without reduction, and osteoarthrosis. Six healthy volunteers served as controls. A pumping procedure was used to take SF from the superior TMJ space. The concentration of lipid peroxidation products was assessed by means of the thiobarbituric acid reaction, and the level of SOD was assessed with spectrophotometry. Results: SF levels of lipid peroxides (LPO) in the control and patient groups were 0.010 (

Purpose: A new operating method was used to treat traumatic temporomandibular joint (TMJ) ankylosis, to restore the structure of the TMJ, to improve the secondary maxillofacial deformity, and prevent recurrence of TMJ ankylosis. Patients and Methods: Thirty-six patients (20 females, 16 males; aged 5 to 54 years old) with TMJ ankylosis type II or III of 1 to 16 years’ duration, with a maximal mouth opening from 0 to 15 mm preoperatively participated. The new method was to separate bony fusion between condyle and glenoid fossa, remove the condylar fragment that displaced medially or roinferiorly, mobilize the remains of the disc over the condylar stump and suture it with rticular capsule, and shave the surface of the condylar stump and glenoid fossa smooth. Results: Follow-up was performed from 1 to 7 years postoperatively in 21 cases. No recurrences occurred in patients whose TMJ disc was retained during operation. Patients had an average maximal mouth opening of 33.7 mm postoperatively. An 11-year-old patient showed an improved facial symmetry after surgery. Conclusion: By restoring the normal structure of the TMJ and preservation of the disc, recurrence of traumatic TMJ ankylosis and facial deformity in younger patients can be prevented.