Morphological changes of CA1 and CA3 pyramidal neurons in rat hippocampus at different intervals following transientforebrain ischemia were examined to determine the nature of post-ischemic cell death in these regions. In the CA1 region, swellingof small dendrites occurred at 24h reperfusion. At 48 h reperfusion, swelling was found in large dendrites of many CA1neurons and the mitochondria and endoplasmic reticulum (ER) were dilated. A small portion of neurons showed chromatinaggregation and nuclear indentation without swelling signs. At 60h reperfusion, swelling of somata was evident in manyneurons. Large dense chromatin clumps with round or ovoid contour were found in other neurons. At 72 and 96 h after ischemia,many large vacuoles and glias with active phagocytosis were observed. At 7 days after ischemia, the tissue was compact and manyglias were found in the region. Most of theCA3 neurons had normal appearance after ischemia. A total of 5-10% CA3 neuronsexhibited shrinking nuclei and chromatin aggregation at 24h reperfusion. The number of these neurons decreased overtime anddisappeared at 72h after ischemia. These results demonstrate the co-existence of necrosis andapoptosis in the CA1 region aftertransient forebrain ischemia. Most CA3 neurons remained intact after ischemia while a small portion of them showed apoptoticcell death.

目的 探讨胶原或明胶吸附施万细胞移植对全横断脊髓结构和功能修复的影响。方法 将胶原或明胶吸附施万细胞移植到成年大鼠全横断脊髓的损伤处，术后3个月用爬网格方式测试动物后肢自主运动功能恢复情况；用荧光金逆行标记法观察大脑感觉运动区和脑干红核的神经元神经纤维再生；用免疫组织化学法检测脊髓CGRP和52HT能神经纤维再生。结果 移植施万细胞的大鼠后肢自主运动功能有显著的恢复。大脑感觉运动区和脑干红核均有被荧光金标记的神经元胞体，提示两个区域的神经元轴突能在脊髓再生并穿越损伤区到达尾侧脊髓。脊髓损伤处有CGRP 和52HT阳性神经纤维，以及损伤处尾侧有52HT阳性神经纤维。对照组大鼠以上结果均为阴性。结论 施万细胞移植可促进大鼠全横断脊髓结构和功能的恢复。胶原或明胶可作为移植细胞的网架用于细胞治疗。

目的 探讨移植神经干细胞对脊髓全横断性大鼠部分结构与功能修复的影响。方法 在脊髓全横断处移植神经干细胞60d 后，在横断处下方3mm注射荧光金逆行标记轴突再生的上运动神经元，用免疫组织化学检测神经干细胞在宿主内的分化，同时用体视学方法观测脑干红核和大脑皮质感觉运动区内锥体细胞层的神经元密度变化。用BBB 评分法和爬网格法观测大鼠后肢运动功能的恢复等。结果 移植的神经干细胞能在宿主内存活并向前后方向迁移到脊髓内，部分神经干细胞分化为GFAP、NF2200和GAP243 阳性细胞。移植神经干细胞后在红核和感觉运动区内锥体细胞层可见有被荧光金标记的神经元胞体，脊髓横断处附近脊髓组织的溃变程度减轻，红核及躯体感觉运动区内神经元密度高于未移植组，大鼠后肢的自主运动功能明显好于未移植组。结论 神经干细胞移植入损伤脊髓后能分化为神经元及神经胶质细胞，能减轻脊髓的继发性损伤，保护受损伤的神经元，促进运动功能的恢复。

神经生长因子（NOF）可促进靶神经节神经突起生长晕的生长。本文应用sH-尿嘧啶核苷和3H一胸腺嘧啶核苷放射自显影术，进一步研究该作用与神经元合成RNA和DNA的关系。用新生大鼠颈上芍，按MaximoW双盖片法进行培养。培养物分NGF组（培养液内添加NGF粗制剂）和对照组（不用NGF）。NGF组培养物被3H一尿嘧啶核苷标记的神经元百分率及标记颗粒水平，均高于对照组，而且每当培养物神经突起生长晕生长速度即将出现高峰时，神经元3H一尿嘧啶核苷标记颗粒水平都明显地增高，说明NGF可促进颈上节神经元的RNA合成，i后者与神经突起的迅速生长有一定相互关系。在3H一胸腺嘧啶核苷标记培养物神经元的实验中，观察到NGF可促进培养第3天的颈上节少量的神经元合成DNA。

Bone marrow mesenchymal stem cells (MSCs) are one of the potential tools fortreatment of the spinal cord injury; however, the survival and differentiation of MSCs in an injuredspinal cord still need to be improved. In the present study, we investigated whether Governor Vesselelectro-acupuncture (EA) could efficiently promote bone marrow mesenchymal stem cells (MSCs)survival and differentiation, axonal regeneration and finally, functional recovery in the transectedspinal cord.