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曾园山, XUEBAO ZHANG , YUANSHAN ZENG , WEI ZHANG , JUNMEI WANG , JINLANG WU , and JUN LI ,
JOURNAL OF NEUROTRAUMA 24(2007)1863-1877,-0001,():
-1年11月30日
Spinal cord transection results in severe neurological sequelae, and to date, there is no effective treatment. Because of the limited capacity for axonal regeneration in the spinal cord, recovery is minimal. Recently, efforts have been made to establish, by grafting neural tissue, a functional relay-station between the severed stumps of the injured cord. Previously, we used co-transplantation of neural stem cells (NSCs) and Schwann cells (SCs) to improve functional recovery of transected spinal cord. However, this effort has been partially impeded by limited neuronal differentiation of transplanted NSCs. To circumvent this problem, we have pre-differentiated NSCs toward neurons in vitro with the application of retinoic acid (RA) prior to cell grafting. Further, we genetically modifiedSCs to overexpress human neurotrophin-3 (hNT-3). When these cells were co-transplanted into the transected spinal cord of rats, injured animals had partial improvement (both functionally and structurally), including improved Basso, Beattie, and Bresnahan (BBB) scores, increased axonal regeneration/remyelination, and reduced neuronal loss. However, this pre-differentiation of NSCs in vitro only mildly improved neuronal differentiation of NSCs in vivo.
adenovirus, co-transplantation, gene therapy, neural stem cells, NT-3,, regeneration, remyelination, retinoic acid, Schwann cells, spinal cord injury
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曾园山, Jiasong Guo a, d, Yuanshan Zeng e, Yuxiang Liang a, Li Wang a, Huanxing Su a and Wutain Wu a, b, c
REGENERATIONANDTRANSPLANTATION Vol.18 No.9 11 June(2007)863-868,-0001,():
-1年11月30日
Cyclosporine is one of the foremost immunosuppressive agents for cell, tissue, and organ transplantation. Cyclosporine is, however, associatedwith signicant side e
cyclosporine,, di
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曾园山, Yuan-Shan Zeng a, Jun-Hui Nie a, Wei Zhang a, Sui-Jun Chen a, Wutian Wu b
BRAINRESEARCH 1130(2007)108-113,-0001,():
-1年11月30日
The present study investigated whether morphine can promote regeneration and synaptic reconstruction of the terminals of injured primary afferent fibers in lamina II of the spinal cord in rats following sciatic nerve injury. Fluoride-resistant acid phosphatase (FRAP)- positive terminals in lamina II of the L4 spinal segment after sciatic nerve injury were assessed after treatment with vehicle, morphine, and naloxone plus morphine. Under the electron microscope, types I and II complex terminals of unmyelinated afferent fibers from the dorsal root, simple terminals of interneuronal axons, and terminals of descending axons at lamina II of the L4 spinal segment were documented in the different groups after injury. FRAP-positive terminals in lamina II were depleted after sciatic nerve injury in the vehicle group. Treatment with morphine increased the numbers of FRAP-positive terminals, and this was prevented by naloxone. The present study demonstrates that morphine may promote the regeneration and synaptic reconstruction of the terminals of injured primary unmyelinated afferent fibers in lamina II of spinal cord, by a process mediated by μ-opioid receptors.
Spinal cord,, Sciatic nerve injury,, Morphine,, Opioid receptor,, Naloxone,, Unmyelinated afferent fiber
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曾园山, J-S Guo , , Y-S Zeng *, H-B Li , W-L Huang , R-Y Liu , X-B Li , Y Ding , L-Z Wu and D-Z Cai
Spinal Cord 45(2007)15-24,-0001,():
-1年11月30日
An animal model oftransected spinal cord injury (SCI) was used to test thehypothesis that cografted neural stem cells (NSCs) and NT-3-SCs promote morphologic andfunctional recoveries of injured spinal cord.Objective: To explore whether cotransplant ofNSC s and NT-3-SCs could promote the injured spinal cord repair. Setting: Zhongshan Medical College, Sun Yat-sen University, PR China. Methods: Female Sprague-Dawley (SD) rats weighing on 200-220g were used to prepare SCI models. The spinal cord was transected between T9 and T10, then NSCs, SCs
neural stem cells, Schwann cells, neurotrophin-3, transplantation, spinal cord injury, regeneration
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曾园山, Jun-Mei Wang a, , Yuan-Shan Zeng a, b, Ran-Yi Liu c, Wen-Lin Huang c, Yi Xiong b, Yan-Hua Wang d, Shui-Jun Chen a, Yang D. Teng e
J.-M. Wang et al./Experimental Neurology 203(2007)123-127,-0001,():
-1年11月30日
AbstractWe have constructed a recombinant adenovirus expression vector carrying the human neurotrophin-3 (NT-3) receptor TrkC (tyrosine proteinkinase C) gene (rAd-TrkC; 2478 bp) and confirmed the expression of the encoded TrkC in green fluorescent protein (GFP)-murine neural stem cells (NSCs) by reverse transcription polymerase chain reaction (RT-PCR),Western blot analysis, andimmunocytochemistry. The activity of the expressedrAd-TrkC was verified in vitro by evaluating dose-related responses of NSCs to NT-3, a TrkC specific ligand. TrkC-GFP-NSCs had a significantly higher percentage of neuronal differentiation when treated with NT-3 relative to the rAd-LacZ control cells (55.2% vs. 29.8%; P<0.05, χ2 test).Thus, our rAd-TrkC vector can transfect NSCs and produce functional TrkC receptors to promote neuronal differentiation of NSCs.
TrkC, Recombinant adenovirus, Gene expression, NT-3, Neural stem cell, Differentiation
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