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鞠熀先, Jiuhong Yu, Songqin Liu, Huangxian Ju*
Biosensors and Bioelectronics 19(2003)401-409,-0001,():
-1年11月30日
A novel amperometric glucose sensor was constructed by immobilizing glucose oxidase (GOD) in a titania sol-gel film, which was prepared with a vapor deposition method. The sol-gel film was uniform, porous and showed a very low mass transport barrier and a regular dense distribution of GOD. Titania sol-gel matrix retained the native structure and activity of entrapped enzyme and prevented the cracking of conventional sol-gel glasses and the leaking of enzyme out of the film. With ferrocenium as a mediator the glucose sensor exhibited a fast response, a wide linear range from 0.07 to 15mM. It showed a good accuracy and high sensitivity as 7.2 μAcm-2 mM-1. The general interferences coexisted in blood except ascorbic acid did not affect glucose determination, and coating Nafion film on the sol-gel film could eliminate the interference from ascorbic acid. The serum glucose determination results obtained with a flow injection analysis (FIA) system showed an acceptable accuracy, a good reproducibility and stability and indicated the sensor could be used in FIA determination of glucose. The vapor deposition method could fabricate glucose sensor in batches with a very small amount of enzyme.
Biosensors, Electrocatalysis, Sol-gel, Titania, Vapor deposition, Glucose, Glucose oxidase, Flow injection analysis
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鞠熀先, Zong Dai, †, Feng Yan, Jin Chen, † and Huangxian Ju*
Anal. Chem. 2003, 75, 5429-5434,-0001,():
-1年11月30日
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鞠熀先, Songqin Liu, Huangxian Ju*
Biosensors and Bioelectronics 19(2003)177-183,-0001,():
-1年11月30日
The direct electrochemistry of glucose oxidase (GOD) adsorbed on a colloidal gold modified carbon paste electrode was investigated. The adsorbed GOD displayed a pair of redox peaks with a formal potential of-(4499±1) mV in 0.1M pH 5.0 phosphate buffer solution. The response showed a surface-controlled electrode process with an electron transfer rate constant of (38.99±5.3)/s determined in the scan rate range from 10 to 100mV/s. GOD adsorbed on gold colloid nanoparticles maintained its bioactivity and stability. The immobilized GOD could electrocatalyze the reduction of dissolved oxygen and resulted in a great increase of the reduction peak current. Upon the addition of glucose, the reduction peak current decreased, which could be used for glucose detection with a high sensitivity (8.4μA/mM), a linear range from 0.04 to 0.28mM and a detection limit of 0.01mM at a signal-to-noise ratio of 3σ. The sensor could exclude the interference of commonly coexisted uric and ascorbic acid.
Biosensors, Carbon paste electrode, Glucose oxidase, Electrocatalysis, Colloidal gold, Glucose
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鞠熀先, Y.K. Ye a, J.H. Zhao b, F. Yan b, Y.L. Zhu a, H.X. Ju a, *
Biosensors and Bioelectronics 18(2003)1501-1508,-0001,():
-1年11月30日
Sequence-known short-stranded hepatitis B virus (HBV) DNA fragment (181 bps) was obtained by PCR method. The strategy for its electrochemical detection was designed by covalently immobilizing single-stranded HBV DNA on gold electrode surface via carboxylate ester as a linkage between 3'-hydroxy end of DNA and carboxyl group of thioglycolic acid (TGA) self-assembled monolayer. The hybridization reaction on surface was evidenced by electrochemical methods using ferrocenium hexafluorophosphate (FcPF6) as an electroactive indicator. The interactions of Fc+with single-stranded (ss) and double-stranded (ds) HBV DNA immobilized on TGA monolayer were studied. The difference between the responses of Fc+at ss-and ds-DNA/Au electrodes suggested that this hybridization biosensor could be conveniently used to monitor DNA hybridization with a high sensitivity. AC impedance and XPS techniques have been employed to characterize the immobilization of ss-DNA on the gold surface.
Hepatitis B virus, PCR, Self-assembled monolayer, Biosensors, Ferrocenium hexafluorophosphate
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鞠熀先, Huang-Xian Ju, a, *, Yong-Kang Ye, Jian-Hua Zhao, b and Yong-Lin Zhu a
Analytical Biochemistry 313(2003)255-261,-0001,():
-1年11月30日
A novel hepatitis B virus (HBV) DNA biosensor was developed by immobilizing covalently single-stranded HBV DNA fragments to a gold electrode surface via carboxylate ester to link the 3'-hydroxy end of the DNA with the carboxyl of the thioglycolic acid (TGA) monolayer. A short-stranded HBV DNA fragment (181 bp) of known sequence was obtained and amplified by PCR. The surface hybridization of the immobilized single-stranded HBV DNA fragment with its complementary DNA fragment was evidenced by electrochemical methods using [Os(bpy)2Cl2]+as a novel electroactive indicator. The formation of double-stranded HBV DNA on the gold electrode resulted in a great increase in the peak currents of [Os(bpy)2Cl2]+in comparison with those obtained at a bare or single-stranded HBV DNA-modified electrode. The mismatching experiment indicated that the surface hybridization was specific. The difference between the responses of [Os(bpy)2Cl2]+at single-stranded and double-stranded DNA/TGA gold electrodes suggested that the label-free hybridization biosensor could be conveniently used to monitor DNA hybridization with a high sensitivity. X-ray photoelectron spectrometry technique has been employed to characterize the immobilization of singlestranded HBV DNA on a gold surface.
Electrochemical DNA biosensor, Polymerase chain reaction, Hepatitis B virus, Hybridization, [Os(, bpy), 2Cl2]
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