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2004年12月28日

【期刊论文】Ca(NO3)2对NaCl胁迫下木麻黄扦插苗生理特征的调控

严重玲, 梁洁, 严重玲*, 李裕红, 张瑞峰, 朱珠

生态学报,2004,24,(5):1073~1077,-0001,():

-1年11月30日

摘要

用不同浓度Ca(NO3)2·4H2O(0.7、1.4、2.1gCa2+/kg土)对1年生的处在两种NaCl胁迫(10和20g/kg)处理下的木麻黄扦插苗进行化学调控,研究硝酸钙盐加氯化钠处理的木麻黄幼苗的生长量、木麻黄幼苗抗氧化酶系统活性和渗透调节渗透调节物质的含量的变化,研究结果表明:中度NaCl胁迫加硝酸钙处理下的木麻黄扦插苗的可深性的蛋白质含量增加,MDA含量降低说明膜脂过氧化作用减轻,而且抗氧化酶活性(SOD、POD)之间协调变化有利于提高清除自由基的速率,中度盐胁迫下钙盐可以促进木麻黄体内脯氨酸的积累;但重度NaCl胁迫下钙盐对木麻黄的调控作用不显著,重度盐胁迫下钙盐反而降低木麻黄SOD、POD活性和脯氨酸的含量,减弱了抗氧化酶系统对活性氧的清除作用,同时高浓度钙盐还会加重NaCl胁迫对木麻黄幼苗的损伤,这说明适量的钙盐有利于木麻黄幼苗抵抗盐胁迫能力的提高,而高浓度钙盐则可能会加重盐胁迫。

NaCl胁迫, 膜保护酶, 脯氨酸, 钙盐调控

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2004年12月28日

【期刊论文】Effects of Rare Earth Elements La and Ce on Ti-Ni Alloy

严重玲, Zhao Yuechao, Ma Zhuang, Fu Dajun, Liu Ailian

JOURNAL OF RARE EARTHS Vol. 21, No.6, Dec. 2003, p. 675,-0001,():

-1年11月30日

摘要

Rare earth elements Ce and La were added into the near-equal atomic titanium-nickel alloy. Differential scanning calorimetry and X-ray diffraction were applied to study the effect of the Ce and La. The results show that adding a small amount of Ce into TiNi alloy can improve the phase transformation obviously, but the phases in TiNi alloy do not change. The results show that adding a small amount of Ce or La can improve the properties of titanium-nickel alloy.

metal materials, TiNi alloy, phase transformation, rare earths

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2004年12月28日

【期刊论文】Effects of Lanthanum on Redox Systems in Plasma Membranes of Casuarina equisetifolia Seedlings Under Acid Rain Stress*

严重玲, Li Yuhong, , Yan Chongling, Liu Jingchun, Mohammed Almasri, Liang Jie, Zhang Ruifeng

JOURNAL OF RARE EARTHS Vol. 21, No.5, Oct. 2003, p. 577,-0001,():

-1年11月30日

摘要

The effects of lanthanum on some redox system (PMRS) properties of the plasma membrane (PM) vesicles from Casuarina equisetifdia seedlings under artificial acid rain (pH4.5) stress were studied. The results show that there are NADH oxidase and EDTA-Fe3+ reductase, and nitrate reductase in the seedling PM, and they have different responses to soaking seeds for 8h in a series of LaCl3 solution. The NADH oxidase activities and the Nitrate reductase activities can be stimulated when La3+ concentrations is in the range of 50~200mg·L-1, but their activities are inhibited or fluctuate by the higher La3+ concentrations. The EDTA-Fe3+ reductase activities can be stimulated by La3+ concentrations in the range of 50~400mg·L-1. The research also revealed that La3+ reduces the relative permeability of membranes and have the function in protecting membranes under acid rain stress by the way of inhibiting the leakage of electrolyte.

ecology, PM redox system, lanthanum, Casuarina equisetifolia, acid rain stress, rare earths

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2004年12月28日

【期刊论文】Partial inhibition of CDK4 gene expression leads to the extension of G1 Phase of V79-8 cells

严重玲, XIAO Weiqun, ZHANG Siqing, WANG Yongchao* and WANG Yue

Chinese Science Bulletin Vol. 44 No.2 January 1999,-0001,():

-1年11月30日

摘要

V79-8 is an abnormal cell line which does not have detectable G1 and G2 phases in its cell cycle. This cell line is derived from V79 cell line which has G1 phase but lacks G2 phase. By using an anti-sense approach, CDK4 gene expression was partially inhibited to find whether CDK4 might contribute to the lack of G1 phase in V79-8 cells. Anti-CDK4 anti-sense plasmid was constructed and used to transfect V79-8 cells. Clones of transfected cells (V79-8-asCDK4) were examined, in comparison with V79-8 cells. to determine its growth curve, cell doubling-time (GT), the level of CDK4 gene expression and the levels of expression of some other growth related genes. V79-8-asCDK4 cells showed a slower growth rate with a doubling time 2.5-h longer than that of V79-8 cells. A flow cytometry (FCM) analysis demonstrated that the 2.5h increse of the doubling time of V79-8-asCDK4 cells was mainly due to the appearance of G1 phase because its G2+ M phase was not significantly different from that of V79-8 cells. The decrease of CDK4 gene expression in V79-8-asCDK4 cells was shown by Northern-blot. Changes in the expression levels of the growth-related genes TGF-

CDK4,, V79-8 cell line,, anti-sense and cell doubling time.,

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2004年12月28日

【期刊论文】碳稳定同位素技术在植物水分胁迫研究中的应用

严重玲, 陈英华, 胡俊, 李裕红, 薛博, 严重玲*

生态学报,2004,24,(5):1027~1033,-0001,():

-1年11月30日

摘要

植物体的碳稳定同位素组成主要由植物本身的生物学特性决定,但环境胁迫对其影响也十分明显。综述了碳稳定同位素技术在研究植物水分利用效率、生物量高低及判断历史气候依据等研究领域的进展,阐明了植物体的δ13C值对干旱、盐分及其它环境因素的变化所引起的水分胁迫的响应,并对碳稳定同位素对水分胁迫的响应机理进行了归纳和推断。

碳稳定同位素, 水分胁迫, 碳同位素比值(, δ13C), , 碳同位素判别(, △), , 水分利用效率(, WUE),

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    厦门大学,福建

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