在研究Fe粉剂量和Fe2+浓度对混合细菌产氢发酵的影响基础上，确定Fe和Fe2+促进混合细菌产氢能力的最佳闽值， 并对乙醇型发酵菌群在不同Fe粉和Fe2+浓度下的产氢量和最大比产氢速率进行考察和对比结果表明，Fe粉和Fe2+对乙醇型发酵菌群的产氢能力均有明显的促进作用以葡萄糖为底物，投加Fe2+试验中，Fe2+浓度200mg/L获得最大产氢量143.7mL/g，较对照组提高32%；Fe2+浓度50mg/L获得单位VSS最大比产氢速率21.2mL/（h·g），较对照组提高33%投加单质Fe试验中，Fe粉剂量1000mg/L获得最大产氢量156.1mL/g，较对照组提高44%；Fe粉剂量500mg/L获得单位VSS最大比产氢速率23.5mL/（h·g），较对照组分别提高47%。单质Fe浓度高于50mg/L时，对发酵菌群产氢的促进作用要优于同浓度下的Fe2+。同时对混合细菌中铁的全量和形态分布进行了考察。

It has been found, in this study, that a new ethanol-type fermentation can be obtained in a continuous flow, high-rate acidogenic reactor receiving molasses as the feed. The operating pH must be maintained at about 4.5 to avoid onset of propionic fermentation. The acidogenic reactor had a VSS level of 20g/L and its organic loading was as high as 80 to 90kg COD/m3d. The operating ORP was around −250mV. The ethanol-type fermentation was characterized by a simultaneous production of acetic acid and ethanol, while the yield of propionic was minimal even at a high organic loading rate of 80 to 90kg COD/m3d, and also, the hydrogen partial pressure was as high as 50kPa. Thus, this study has shown that the production of propionic acid is not always related to high hydrogen partial pressure. When the operating pH was increased to 5.5, the yield of propionic acid became significant.