Preparation and evaluation of BC@Laccase subtractive materials for chlorophenolic organics

• 1、College of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, China
• 2、College of Science, Beijing forestry University, Beijing 100083, China
• 3、Tianjin Changwei Biotech Co. Ltd, Tianjin 300300, China

Abstract：To solve the problems of poor stability and low reuse rate of free laccase, it was fixed on bacterial cellulose (BC) substrate by glutaraldehyde crosslinking method. By optimizing the preparation process, the environmentally friendly and recyclable BC@Laccase chlorophenol organic subtractive material was prepared. It was characterized by scanning electron microscope, fourier transform infrared spectrometer and thermogravimetric analyzer. This material was applied to the treatment of 2-chlorophenol. The results showed that the best BC@Laccase subtractive materials were prepared at the special condition that the reaction time was 4 h, reaction temperature was 25 ℃ and reaction pH value was 3.5. The BC@Laccase subtractive material was used to degrade 2-chlorophenol under the optimal conditions. The best degradation effect was obtained at the medium dosage was 4 mM, the treatment temperature was 30 ℃, the pH value was 3, and the treatment time was 5 h. At this condition, the degradation rate reaching 76.9%.

Keywords： bacterial cellulose immobilization laccase medium 2-chlorophenol