Breeding of High Arginine Deiminase Producing Strain by Compound Mutagenesis and Its Fermentation Process Optimization

• 1、State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122

Abstract：To improve the arginine deiminase producing ability, Enterococcus faecalis SK 32.001 was mutated using chemical method coupled with atmospheric room temperature plasmas. A high arginine deiminase producing strain A3 was obtained by plate initial screening and re-screening in shake flask fermentation. Then the enzyme producing ability of the strains was further improved by optimizing fermentation conditions. The results showed that the enzyme activity of the mutant strain A3 was 3.12 U/mL with sucrose and beef extract as carbon and nitrogen sources, respectively, and with the dissolved oxygen value was controlled at 20%. Compared with the original strain, the enzyme activity was improved by 162.18%.

Keywords： Enterococcus faecalis arginine deiminase chemical mutagenesis atmospheric room temperature plasmas