Analysis of rice cell wall related proteins and their interaction networks

• 1、State key laboratory of conservation and utilization of biological resources, yunnan agricultural university, kunming 650201, China

Abstract：With the rapid development of research techniques, the structure and function of plant cell walls have been increasingly understood. The analysis of cell wall related proteins in the whole rice genome by various bioinformatics methods can provide important clues and basis for exploring the characteristics and functions of these proteins which are difficult to isolate and purify. In this study, cell wall related proteins were retrieved from the latest version of the rice genome database. Among them, the enzymatic proteins included cell wall related receptor kinases (WAKs), arabinogalactose proteins (AGPs) and cellulosic metabolic enzymes (CMEs), while the non-enzymatic proteins included nitrate transporter 1 and peptide transporter protein (NPFs) and glycine-rich cell wall structural proteins (GRPs). The results of intra - and inter-group protein interaction analysis showed that there were significant differences in interaction patterns among different group of proteins. There was no interaction within both WAKs and GRPs proteins, and there were interactions within AGPs and DMEs. The interactions among proteins in the NPFs are the most intense. There was also a close interaction among proteins of AGPs and CMEs. To combine with the rice disease-resistance regulatory gene database, it was found that there were regulatory proteins related to disease resistance in all the above groups, many of which were non-host resistant proteins, indicating that many of the disease-resistance mediated by cell wall related proteins belonged to non-host resistance. These results established a foundation for further experiments to explore the functions of these proteins for cell walls and disease resistance.

Keywords： Rice cell wall-related proteins functions interaction networks disease resistance regulation protein