EYcient production, puriWcation, and application of egg yolk antibodies against human HLA-A*0201 heavy chain and light chain (β2m)
Protein Expression and PuriWcation 44(2005)45-51，-0001，（）：
The importance of eggs as a source of speciWc antibodies is well recognized. Egg yolk contains 8-20 mg immunoglobulins (IgY) per milliliter. However, the major problem in separating IgY is to remove the high concentrations of lipids in egg yolk. We Wrst used water dilution method to get the supernatant containing IgY, then puriWed the antibody by caprylic acid–ammonium sulfate method, and obtained speciWc antibody with satisfactory purity and activity. By comparison of these several methods, each has its advantages, one can be chosen to purify IgY according to practical need. The puriWed IgY produced by the immunized chickens can stain the human peripheral blood mononuclear cell eVectively when labeled with Xuorescent FITC.