Staphylococcus epidermidis strains are diverse in their pathogenicity; some are invasive and cause seri-ous nosocomial infections, whereas others are non-pathogenic commensal organisms, To analyse the implications of different virulence factors in Staphylo-coccus epidermidis infections, the complete genome of Staphylococcus epidermidis strain ATCC 12228, a non-biofilm forming, non-infection associated strain used for detection of residual antibiotics in food prod-ucts, was sequenced, This strain showed low viru-lence by mouse and rat experimental infections, The genome consists of a single 2499 279 bp chromosome and six plasmids. The chromosomal G+C content is 32.1% and 2419 protein coding sequences (CDS) are predicted, among which 230 are putative novel genes. Compared to the virulence factors in Staphylococcus aureus, aside from δ-haemolysin and 13-haemolysin, other toxin genes were not found. In contrast, the majority of adbesin genes are intact in ATCC 12228. Most strikingly, the ica operon coding for the enzymes synthesizing interbacterial cellular polysaccharide is missing in ATCC 12228 and rearrangements of adja-cent genes are shown. No mec genes, IS256, IS257, were found in ATCC 12228. It is suggested that the absence of the ica operon is a genetic marker in com-mensal Staphylococcus epidermidis strains which are less likely to become invasive.