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期刊论文
Construction of DNA vaccines and their induced protective immunity against experimental Eimeria tenellainfection
Parasitol Res (2004) 94: 332-336，-0001，（）：
In an attempt to construct a DNA vaccine against chicken coccidiosis, the TA4 gene of Eimeria tenella strain BJ was ligated to the mammalian expression vector pcDNA3.1/Zeo (+) to give pcDNA3.1-TA4 (pcDT). Then, Et1A (E. tenella refractile body gene) was ligated to it, upstream, aiming to be expressed in fusion with TA4, giving pcDNA3.1-Et1A-TA4 (pcDET). The constructed DNA vaccines were given to broilers intramuscularly 10-15min after the breasts had been pretreated with 25% sucrose solution. At 7 days after the second vaccination, chickens were challenged with 3
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