Ethylglucoside monooleate was synthesized by esteriication between ethylglucoside and oleic acid with immobilized lipase from Candida antarctica in a solvent-free system. It was shouwn that a stirred tnk reactor was suitable for the enzymatic reaction process involving substrates with low miscibiligy, in which the biocatalyst was recycled five times without signiicant activity loss. Removal of the co-product, water, from thereactin medium by carrying out the reaction under reduced pressure benefited the esterification reaction and increased the monooleate yield up to 97% within 8 hours.