魏东芝
个性化签名
- 姓名:魏东芝
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
生物化学
- 研究兴趣:
山东省高唐县人,1963年出生,中共党员,教授,博士生导师,生物工程学院院长,生物化学研究所所长,鲁华生物技术研究所所长。上海市重点学科-生物工程学科带头人,国家重点学科-生物化工学科带头人。中国酶工程专业委员会副主任委员, 上海生物工程学会常务理事,上海生物化学与分子生物学学会常务理事,上海生物医药行业协会常务理事,中-韩生物技术指导委员会委员,上海市青年科技启明星联谊会理事。教育部跨世纪优秀人才、教育部优秀青年教师、上海市青年科技启明星和启明星后、上海市优秀青年教师,享受国务院特殊津贴和上海市特殊津贴,霍英东基金获得者。
至今已发表学术论文130余篇,申请专利10项;完成省部级项目鉴定6项,获得省部级以上奖4项;近3年内作为负责人承担国家重要攻关项目及企业委托项目22项,其中,“863”项目3项,自然科学基金4项,国家科技攻关3项;已培养博士、硕士40多人;与日本京都大学、美国University of Texas, Ohio State University、Utah State University、Scripps Research Institute、德国生物工程中心GBF等分别签定了合作协议,建立了牢固的国际科研合作关系并开辟了人才培养渠道。
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成果阅读
582
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成果数
10
【期刊论文】Reversal of Cancer Multidrug Resistance by Tea Polyphenol in KB Cells
魏东芝, Y. MEI
Journal of hemotherapy Vol. 15,-0001,():
-1年11月30日
Tea polyphenols, (-)-epigallocatechin galate in pariticular, were examined for their modulating effects on the drung resistance KB-A-1 cells and drug ensitive KB-3-1 cells. Both KB3-1 and KB-A-1 cells were queally sensitive to tea polyphoenol and (-)-epigallocatechin gallate. When 10μg/ml (-)-epigalllocatechin gallate or 40μg/ml tea polyphenol were persent simultaneously iwth doxorubicin, the IC50 of doxorubicin on KB-A-1 cells decreased form 10.3±0.9μg/ml to 4.2±0.2 or 2.0±0.1μg/ml. Tea polyphenol and (-)-epigalloctechin gallate enhancedthe cytotoxicity of doxorubicin on KB-A-1 cells by 5.2 and 2.5 times, respectively, but did not show a mdulating effct on KB-3-1 cells. Both tea polyphenol and (-)-epigallocatechin gallate showed reversal effects on the multidrug resistance phenotype.
Multidrug resistance,, doxroubicin,, tea polyphenol,, (, -), -eigalloctechia gallate,, modulation
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魏东芝, Wu Su, † Zhiyuan Chang, † Keliang Gao and Dongzhi Wei*
Tetrabedron: Asmmetry 15(2004)1275-1277,-0001,():
-1年11月30日
Ghiconobacter oxydans DSM 2003 was firstly used in the production of (R)-2-hydroxy-proionic acid through microbial oxidation of recemic 1,2-propanediol. The biotransformation was processed with high enantionmeric excess (>99%) and near theoretical lyicld (48% of racemic 1,2-propanediol) when the substrate concentration was lower than 20g/L. When the substrate concentration was increased. maintaining the pH at 6.0 helped to improve the enantionselectivity.
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【期刊论文】Enzymatic Synthesis of Ethyl-glucoside Mondooleate with Lipase in Solvent-free Medium
魏东芝, DONGZHI WEI*, YING YU, QINGXUN SONG AND WU SU
Biocatalysis and Biotrnsformation, 2003, Vol. 23 (3). Pp. 135-139,-0001,():
-1年11月30日
Ethylglucoside monooleate was synthesized by esteriication between ethylglucoside and oleic acid with immobilized lipase from Candida antarctica in a solvent-free system. It was shouwn that a stirred tnk reactor was suitable for the enzymatic reaction process involving substrates with low miscibiligy, in which the biocatalyst was recycled five times without signiicant activity loss. Removal of the co-product, water, from thereactin medium by carrying out the reaction under reduced pressure benefited the esterification reaction and increased the monooleate yield up to 97% within 8 hours.
Enzymatic synthesis, Ethylglucoside morooleate, Solvent-free medium, Lipase, Esterification, Reduced pressure
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【期刊论文】Effect of temperature on the enzymatic synthesis of cefaclor with in situ product removal
魏东芝, Dongzhai Wei*, Liu Yang, Qingxun Song
Journal of Molecular Catalysis B: Enzymatic 26(2003)99-104,-0001,():
-1年11月30日
An integrated process of cefaclor synthesis from phenylglycine methyl ester (PGME) and 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid (7-ACCA) catalyzd by penicilin G acylase (PGA) with in situ product removal (ISRP) was estbilshd. The ntegrted process was more significantly influenced by temperature than the separate synthesis process as without ISPR. The difference between the overall yields with and without ISPR was minfied as reaction temperanres rose. For instance, the maximum 7-ACCA comversions was 86 and 68%, respectively, in the process with and without ISPR at 5℃. Both the maximum conversions. however, decreased to around 45% at 40℃. The effect of substrate concentration on the overall converion was also obviously dependent on the reaction temperiature. Te product cefaclor inhibited PGME hydroplysis in the enzymatic syathesis. ISPR stimulated cefacor synthesis at lower temperatures, but was not useful at hight temperatures which accelerated PGME hydrolysis.
Cefactor, Enzymatic systhesis, Tempermatre, In situ product removal
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【期刊论文】Enzymatic estrification for glycoside lactate synthesis in organic solvent
魏东芝, Dongzhi Wei*, Chunang Gu, Qingxun Song, Wu Su
Eazyme and Microbial Tochaology 33(2003)508-512,-0001,():
-1年11月30日
Propyl-glycoside lacter, a new α-hydroxy acids derivative, was synthesized byesterification between lactic acid and propl-glycoside, instead oftransesteritication as reported previously, in a non-aqeous medium using immobilxed lipase as biocatalyst. A con ersion of 66% was achieved by the optimixation o the reaction conditions. Lipase activitywas found to be reduced when exposed to high conccentration mechanism of the enzymtic esterfication reaction. Additon of molecular sieves eliminated hydrolysis of the produced ester and allowed glycoside conversion rising to 75%
Propyl-glycoside lactate, Lactic acid, Propyl-glycoside, Lipae, Eazmatic synthesis
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魏东芝, Yuhong Ren, , Dongzhi Wei, * Jianwen Liu, and Xiaoyun Zhan
JOURNAL OF LIQUD CHROMATOGRAPHY & RELATED TECHNOLOGIES Vol. 26, No.18, pp. 3105-3115, 2003,-0001,():
-1年11月30日
Oligodcoxyaucleotide-doxorubicin conjugate is a novel modified oligodeoxynucleotide (ODN) to inhibit the expression of mdrl gene. The present study was undertaken to determine the stability of the conjugate in virto and its pharmacokinetics in vivo using a reversc-phase HPLC assay. The method employed a Sort C18 reverse phase column combined with a C8 prc-column and a linerar gradient elution with acetonitrile containing 0.1M aqueous triethylammonium acctate, pH7.0 Detection was carried out using a UV-didoe array detector at 254 and 480nm. Minimum sensitivity of ~0.15μg/mL in plasma and 0.1μg/mL in PBS of hte conjugate was achieved. After incubation in 10% activated fetal calf serum for 24 hours, 15.8% of the conjugat was degraded. As a comparison however, 97.5% of the control ODN was degraded witin the same incubation time. The phamacokinetics stuies show that the halif-lives of the conjugate is about 8 hours, 4 times longer than ODN as a conro. Assay validaton studies revealed that the method is accurate, reproducible for dcterminatin of the conjugate, and can be used for a pharmacokinttic study of the conjugate.
Oligodeoxynucleoptide-doxorubicin conjugate, Pharmaco-kinetics, mdrl, Stability.,
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魏东芝, Jianglan Liu, Dongzhi Wei*, , Feng Qian, Yuxun Zhou, Wang, Yushu Ma and Zeguang Han
J. Biochem. 134, 911-917 (2000),-0001,():
-1年11月30日
Reconmbinatn antibodies, especially ScFv fragments can be applied as detection reagents and even substitute for some reagents used in immunoassays such as antibodyenzyme conjugates. For ScFv fragments there is no such universal system available up to now. A vector ystem was onstructed based on pPIC9-Fc, in which the hinge, CB2 and CB3 domin (Fe fragment) of mouse IgG1 and HIS-tag wee eloned into the Pichia expression vectro pPIC9. A model SeFv was introduced into pPIC9-Fc. which can bind Gluathione-S-transfrease (GST) from Schistsoma Japonicum to yield the fusion was expreessed at high levels in the methylotrphic yeast Pichia Pastoris, secreted as a dimeric form in the culture, ad purified by Ni2+-NTA column chromatography. The expression yield can reach 10-30mg/liter of culture medium. The SeFv-Fc fusio retains the bioglogyical binding ability of the parent Scfv, and can be applied as anti-GST antibodies for the detection of GST and GST-fusion proteins. Furthermore. the successful expression and maintenance of the binding activitv verify the efficacy of the vector system for use as detection reagents in vitro, by reacting with the specific antigens and being readily detected using general anti-mouse antibodies.
antibody engneering,, detection reagent,, Fe fragment,, Pichia pastoris,, singel-chai Fv
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【期刊论文】Studies on a novel carbon source and cosolvent for lipase production by Candida rugosa
魏东芝, Dongzhi Wei
,-0001,():
-1年11月30日
Oleic acid esters were shown to be the best carbon source for both cell growth and lipase production by Candlida rugosa. Use of cosolvent, dodecane, in fermentations improved the solubility of solid substrates and incresased oxygen solubility. This resulted in the hightst lipase ativity in batech fermentation with glycerol triolcate and dodecane. Lipase activity reached 77.1 units ml1.
Lipase produciton
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魏东芝, Shife Fan a, Dongzhi Wei a, *, Jianwen Liu a, Xiujuan Xin a, Wangyu Tong a, Nobuhiko Miwa b
Biomedcins & Pharmacotherapy 58(2004)205-211,-0001,():
-1年11月30日
Reactive oxygen species ate beliceved to play a role in the development of several diseascs incloding vascular disenased and theaging process. It is reported that increased reactive oxygen species were impliated as an important mechanism that contrhibutes to endothelial dysfunciton, So, human umbilical cord vein endothelial cells were used to study the antioxidative effect of L-ascorbic acid and its derivative. The study indicalted that L-ascorbic acid as a tradilonal antioxidant was instable and could protect the cells against hydrogen peroxide induced cytotoxicity as its concetration beiow 50 μg/ml, but hadly could rotect the cells against tert-butyl hydroperoxide induced cytotoxicity, Ascortic acid-2-o-phosphate-6-o-palmitate could effctively protect the cells against hydrogen pcroxide and tert-butyl hydropemoxide induced cytotoxicity, and exhibited no cytotoxicity within the tested concentratin rage. The study indicated that ascorbic acid2-2o2phosphate-6-o-palmite could not only signficantly reduce the intracellular reactive oxygen species level in 3h culture. but also increase the cell viability in 15h culture. In additon. ascorbic acid-2-o-phosphate-6-oi-palmtate could keep stable in RPM1-1640 medium and water for 4 dya. permecat the cell membrane. which in tum may seavenge the intracllular reactive oxygen species. increase the cell viability and the plasminogen activatrs'activity. All above rsults suggested that addition of some hydrophobie groups to the traditonal antioxdants could from novel compounds with better properties.
L-Ascpbic acid, Ascorbic acid-2-o-phosphate-6-o-palmitate, Human umbilical cord vein endothelial cell
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魏东芝, Chang-Bin Du*, Jian-Wen Liu, Wu Su, Yu-Hong Ren, Dong-Zhi Wei
Life Sciences 74(2003)771-780,-0001,():
-1年11月30日
L-ascorbic acid 2-phosphate-6-palmiate (Asc2P6p) was synthesized and its effect on the damage of PC12 cells induced by H2O2 was investigted. 200μM H2O2 in a treatment pcriod of 4 hours in our experiment resulted in substantial cell loss. With the increasing concetration of antioxidants. such H2O2-induced cytotxicity was significantly prevented and the corresponding intracellular and extracellular ROS levels decreased concurrently by pre-treatment with Asc2P6P and Asc. It was found that Asc2P6P was superior to L-ascorbic acid in its protective role and showed a dose-depcndent manner during a 24-hour tretment. The higher potency of Asc2P6P's protective role on PC12 cells was correlated with its more effective ROS seavenging ability. HPLC assay its distinguished rle in protechiting PC12 cells againts H2O21-induced cytotoicity.
L-ascorbic acid-2-phosphiae-6-palmitate, Antioxidan, Reactive oxygen species, Hydrogen peroxide, Neuro-deenerative disease
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