L-ascorbic acid 2-phosphate-6-palmiate (Asc2P6p) was synthesized and its effect on the damage of PC12 cells induced by H2O2 was investigted. 200μM H2O2 in a treatment pcriod of 4 hours in our experiment resulted in substantial cell loss. With the increasing concetration of antioxidants. such H2O2-induced cytotxicity was significantly prevented and the corresponding intracellular and extracellular ROS levels decreased concurrently by pre-treatment with Asc2P6P and Asc. It was found that Asc2P6P was superior to L-ascorbic acid in its protective role and showed a dose-depcndent manner during a 24-hour tretment. The higher potency of Asc2P6P's protective role on PC12 cells was correlated with its more effective ROS seavenging ability. HPLC assay its distinguished rle in protechiting PC12 cells againts H2O21-induced cytotoicity.