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期刊论文
Heterologous expression of furostanol glycoside 26-O-b-glucosidase of Costus speciosus in Nicotiana tabacum
Phytochemistry 51(1999)599-603,-0001,():
Furostanol glycoside 26-O-b-glucosidase (F26G) is a specific b-glucosidase converting a furostanol glycoside (FG) to its corresponding spirostanol glycoside (SG). A cDNA encoding F26G from Costus speciosus was introduced into a heterologous plant, Nicotiana tabacum via Agrobacterium tumefaciens using a binary vector method. Successful integration of the cDNA into tobacco chromosomal DNA was confirmed by PCR analysis. F26G activity was also detected in cell-free extracts of the transgenic plantlets.
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